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Literature summary for 3.1.4.37 extracted from

  • Tyc, K.; Kellenberger, C.; Filipowicz, W.
    Purification and characterization of wheat germ 2,3-cyclic nucleotide 3-phosphodiesterase (1987), J. Biol. Chem., 262, 12994-1300.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
Triticum aestivum
Triton X-100
-
Triticum aestivum

General Stability

General Stability Organism
freezing and thawing, not affected by several cycles Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 74% inhibition at 0.5 mM Triticum aestivum
Cu2+ 95% inhibition at 0.5 mM Triticum aestivum
Fe2+ 33% inhibition at 0.5 mM Triticum aestivum
Fe3+ 66% inhibition at 0.5 mM Triticum aestivum
NaCl
-
Triticum aestivum
PO43-
-
Triticum aestivum
Zn2+ 82% inhibition at 0.5 mM Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Triticum aestivum
9.2
-
2',3'-cGMP
-
Triticum aestivum
13.1
-
2',3'-cAMP
-
Triticum aestivum
25.2
-
2',3'-cCMP
-
Triticum aestivum
25.3
-
2',3'-cUMP
-
Triticum aestivum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight stimulation Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
gel filtration Triticum aestivum
24000
-
x * 24000, SDS-PAGE Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
16000fold to near homogeneity Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
280
-
2',3'-cGMP Triticum aestivum
600
-
2',3'-cUMP Triticum aestivum
2090
-
2',3'-cAMP Triticum aestivum
2140
-
2',3'-cCMP Triticum aestivum

Storage Stability

Storage Stability Organism
-70°C, stable for at least 5 months Triticum aestivum
4°C, Tris-acetate buffer, 0.5 mM dithiothreitol, pH 7.6, 10% glycerol, protease inhibitor mix, 3 days Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
Triticum aestivum 2'-AMP
-
?
2',3'-cCMP + H2O
-
Triticum aestivum 2'-CMP
-
?
2',3'-cGMP + H2O
-
Triticum aestivum 2'-GMP
-
?
2',3'-cUMP + H2O
-
Triticum aestivum 2'-UMP
-
?
additional information not: 2',3'-cyclic esters in cyclic phosphate-terminated oligoribonucleotides or in nucleoside 5'-phosphate, 2',3'-cyclic phosphate Triticum aestivum ?
-
?
nucleoside 2',3'-cyclic phosphate + H2O
-
Triticum aestivum nucleoside 2'-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Triticum aestivum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
no loss of activity after 10 min Triticum aestivum
65
-
20% loss of activity after 10 min Triticum aestivum
80
-
82.2% loss of activity after 10 min Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Triticum aestivum

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Triticum aestivum