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Literature summary for 3.1.4.37 extracted from

  • Raasakka, A.; Myllykoski, M.; Laulumaa, S.; Lehtimaeki, M.; Haertlein, M.; Moulin, M.; Kursula, I.; Kursula, P.
    Determinants of ligand binding and catalytic activity in the myelin enzyme 2,3-cyclic nucleotide 3-phosphodiesterase (2015), Sci. Rep., 5, 16520 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic domain, sittging drop vapor diffusion method, using 20-35% (w/v) PEG3350, PEG4000, or PEG6000 as precipitants and 50 mM Na acetate or 100 mM Na citrate, pH 3.0-5.0 Mus musculus

Protein Variants

Protein Variants Comment Organism
F235A the mutant shows near-complete catalytic inactivation Mus musculus
F235L the mutant shows near-complete catalytic inactivation Mus musculus
H230Q inactive Mus musculus
H230Q/H309Q inactive Mus musculus
H230S inactive Mus musculus
H309Q inactive Mus musculus
H309S the mutant shows strongly reduced activity compared to the wild type enzyme Mus musculus
P225G the mutant shows increased activity compared to the wild type enzyme Mus musculus
P296G the mutant shows slightly increased activity compared to the wild type enzyme Mus musculus
R307Q the mutant shows reduced activity compared to the wild type enzyme Mus musculus
T232A the mutant shows near-complete catalytic inactivation Mus musculus
T311A the mutant shows near-complete catalytic inactivation Mus musculus
V321A the mutant shows reduced activity compared to the wild type enzyme Mus musculus
Y168A the mutant shows near-complete catalytic inactivation Mus musculus
Y168S the mutant shows reduced activity compared to the wild type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.483
-
 2',3'-cNADP+ mutant enzyme P225G, pH and temperature not specified in the publication Mus musculus
0.553
-
 2',3'-cNADP+ wild type enzyme, pH and temperature not specified in the publication Mus musculus
0.722
-
 2',3'-cNADP+ mutant enzyme P296G, pH and temperature not specified in the publication Mus musculus
1.045
-
 2',3'-cNADP+ mutant enzyme V321A, pH and temperature not specified in the publication Mus musculus
1.055
-
 2',3'-cNADP+ mutant enzyme H230Q, pH and temperature not specified in the publication Mus musculus
1.192
-
 2',3'-cNADP+ mutant enzyme H309S, pH and temperature not specified in the publication Mus musculus
1.305
-
 2',3'-cNADP+ mutant enzyme H230S, pH and temperature not specified in the publication Mus musculus
1.385
-
 2',3'-cNADP+ mutant enzyme Y168S, pH and temperature not specified in the publication Mus musculus
1.879
-
 2',3'-cNADP+ mutant enzyme Y168A, pH and temperature not specified in the publication Mus musculus
2.192
-
 2',3'-cNADP+ mutant enzyme R307Q, pH and temperature not specified in the publication Mus musculus
3.788
-
 2',3'-cNADP+ mutant enzyme F235L, pH and temperature not specified in the publication Mus musculus
6.253
-
 2',3'-cNADP+ mutant enzyme F235A, pH and temperature not specified in the publication Mus musculus
39.648
-
 2',3'-cNADP+ mutant enzyme T232A, pH and temperature not specified in the publication Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside 2',3'-cyclic phosphate + H2O Mus musculus
-
 nucleoside 2'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P16330
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
 Mus musculus 2'-AMP
-
 ?
2',3'-cCMP + H2O
-
 Mus musculus 2'-CMP
-
 ?
2',3'-cNADP+ + H2O
-
 Mus musculus NADP+
-
 ?
nucleoside 2',3'-cyclic phosphate + H2O
-
 Mus musculus nucleoside 2'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
2',3'-cyclic nucleotide 3'-phosphodiesterase
-
 Mus musculus
CNPase
-
 Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
 melting temperature Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 2',3'-cNADP+ mutant enzyme H230S, pH and temperature not specified in the publication Mus musculus
21
-
 2',3'-cNADP+ mutant enzyme H309S, pH and temperature not specified in the publication Mus musculus
24
-
 2',3'-cNADP+ mutant enzyme H230Q, pH and temperature not specified in the publication Mus musculus
183
-
 2',3'-cNADP+ mutant enzyme F235A, pH and temperature not specified in the publication Mus musculus
221
-
 2',3'-cNADP+ mutant enzyme F235L, pH and temperature not specified in the publication Mus musculus
231
-
 2',3'-cNADP+ mutant enzyme T232A, pH and temperature not specified in the publication Mus musculus
237
-
 2',3'-cNADP+ mutant enzyme Y168A, pH and temperature not specified in the publication Mus musculus
732
-
 2',3'-cNADP+ mutant enzyme V321A, pH and temperature not specified in the publication Mus musculus
887
-
 2',3'-cNADP+ mutant enzyme Y168S, pH and temperature not specified in the publication Mus musculus
940
-
 2',3'-cNADP+ wild type enzyme, pH and temperature not specified in the publication Mus musculus
1182
-
 2',3'-cNADP+ mutant enzyme R307Q, pH and temperature not specified in the publication Mus musculus
1332
-
 2',3'-cNADP+ mutant enzyme P296G, pH and temperature not specified in the publication Mus musculus
1420
-
 2',3'-cNADP+ mutant enzyme P225G, pH and temperature not specified in the publication Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 2',3'-cNADP+ Kcat_Km less than 10 1/sec*mM, mutant enzyme T232A, pH and temperature not specified in the publication Mus musculus
10
-
 2',3'-cNADP+ mutant enzyme H230S, pH and temperature not specified in the publication Mus musculus
20
-
 2',3'-cNADP+ mutant enzyme H230Q, pH and temperature not specified in the publication Mus musculus
20
-
 2',3'-cNADP+ mutant enzyme H309S, pH and temperature not specified in the publication Mus musculus
30
-
 2',3'-cNADP+ mutant enzyme F235A, pH and temperature not specified in the publication Mus musculus
60
-
 2',3'-cNADP+ mutant enzyme F235L, pH and temperature not specified in the publication Mus musculus
130
-
 2',3'-cNADP+ mutant enzyme Y168A, pH and temperature not specified in the publication Mus musculus
540
-
 2',3'-cNADP+ mutant enzyme R307Q, pH and temperature not specified in the publication Mus musculus
640
-
 2',3'-cNADP+ mutant enzyme Y168S, pH and temperature not specified in the publication Mus musculus
700
-
 2',3'-cNADP+ mutant enzyme V321A, pH and temperature not specified in the publication Mus musculus
1700
-
 2',3'-cNADP+ wild type enzyme, pH and temperature not specified in the publication Mus musculus
1730
-
 2',3'-cNADP+ mutant enzyme P296G, pH and temperature not specified in the publication Mus musculus
2940
-
 2',3'-cNADP+ mutant enzyme P225G, pH and temperature not specified in the publication Mus musculus