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(25R)-3beta-hydroxycholest-5-en-27-oate + H2O
?
-
-
-
-
?
(glucose)4 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)2 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)5 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)6 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(xylose)2 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(xylose)5 + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
1-naphthyl acetate + H2O
naphthol + acetate
1-naphthyl butanoate + H2O
1-naphthol + butanoate
no substrate for wild-type
-
-
?
1-naphthyl caprate + H2O
1-naphthol + caprate
-
-
-
?
1-naphthyl myristate + H2O
1-naphthol + myristate
-
-
-
?
1-naphthyl octanoate + H2O
1-naphthol + octanoate
no substrate for wild-type
-
-
?
1-naphthyl propionate + H2O
1-naphthol + propionate
-
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
2,3,4-tri-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective double deacetylation, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2,3,4-tri-O-acetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2,4-di-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2,4-di-O-acetyl-methyl beta-D-xylopyranoside + H2O
?
-
-
-
?
2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside + H2O
2-deoxy-2-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside + H2O
2-deoxy-4-O-acetyl beta-D-xylopyranoside + 2-deoxy-3-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
acetylated xylopyranosides in ratio 2:1
?
2-naphthyl acetate + H2O
2-naphthol + acetate
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
acetate + 4-nitrophenyl-beta-D-xylopyranoside
3,4-di-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
3,4-di-O-acetyl-methyl beta-D-xylopyranoside + H2O
?
-
-
-
?
3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside + H2O
3-deoxy-3-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
3-naphthyl acetate + H2O
3-naphthol + acetate
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
4-nitrophenyl glucopyranoside + H2O
4-nitrophenol + glucose
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
?
4-nitrophenyl xylopyranoside + H2O
4-nitrophenol + xylose
-
-
-
-
?
4-O-methyl-D-glucuronosyl-alpha-1,2-D-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylopyranose + H2O
4-O-methyl-D-glucuronosyl-alpha-1,2-D-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylopyranose + beta-1,4-xylopyranosyl-beta-1,4-xylopyranose
-
-
-
-
?
4-O-methyl-glucuronoxylan + H2O
? + acetate
-
main hemicellulose in deciduous trees, and contains beta-(1,4)-linked xylopyranosyl backbone units that can be acetylated and/or substituted by alpha-(1,2)-linked 4-O-methylglucopyranosyluronic acid
-
-
?
7-amino cephalosporanic acid + H2O
? + acetate
-
-
-
-
?
7-aminocephalosporanic acid
acetate + ?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
?
acetyl-xylobiose
acetate + ?
-
very low activity
-
-
?
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
acetylated birchwood xylan + H2O
acetate + birchwood xylan
-
-
-
?
acetylated birchwood xylan + H2O
deacetylated birchwood xylan + acetate
acetylated chitin + H2O
acetate + chitin
enzymatic deacetylation of chitinous substrates by AxeA is influenced by both substrate accessibility and mode of enzyme action. Barely acts on solid pure chitin whatever its form: native alpha-chitin and whiskers or native beta-chitin. But when chemically treated in strong alkali, chitin is partially deacetylated. The longer the alkali treatment, the more efficient the deacetylation of alkali-treated chitins
-
-
?
acetylated chitosan + H2O
? + acetate
acetylated colloidal chitin + H2O
? + acetate
acetylated glycol chitin + H2O
? + acetate
acetylated xylan + H2O
partially deacetylated xylan + acetate
acetylated xylan + H2O
xylan + acetate
acetylated xylooligosaccharide + H2O
?
acetylated xylooligosaccharides + H2O
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
alpha-naphthyl acetate
acetate + 2-naphthol
alpha-naphthyl acetate + H2O
1-naphthol + acetate
alpha-naphthyl propionate + H2O
1-naphthol + propionate
alpha-terpinyl acetate + H2O
alpha-terpineol + acetate
-
-
-
-
?
amyl acetate
acetate + amyl alcohol
-
-
-
-
?
an acetic ester + H2O
an alcohol + acetate
aspen acetyl glucuronoxylan + H2O
?
beechwood acetyl glucuronoxylan + H2O
?
beta-D-xylose tetraacetate + H2O
?
beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc + H2O
?
-
native AxeA and two variants display about 50% deacetylation of the oligohexamer substrate after reaction at 50°C for 24 h in diluted culture supernatant. Characterization of the deacetylation products from reaction of wild-type and mutant AXEs, overview
-
-
?
beta-naphthyl propionate + H2O
2-naphthol + propionate
birch acetyl glucuronoxylan + H2O
?
-
positional specificity of the enzyme
-
-
?
birchwood acetyl glucuronoxylan + H2O
?
birchwood xylan + H2O
?
-
-
-
?
butyl acetate
acetate + butanol
-
-
-
-
?
cefotaxime + H2O
? + acetate
-
-
-
-
?
cellobiose octaacetate + H2O
?
-
-
-
?
cellobiose octaacetate + H2O
? + acetate
-
-
-
-
?
cellulose acetate
acetate + ?
-
-
-
-
?
cellulose acetate + H2O
?
different samples, CA0.88 is the best substrate, efficient complete degradation when the enzyme is mixed with a cellulolytic endo-1,4-beta-glucanase, EC 3.2.1.4
-
-
?
cellulose acetate + H2O
? + acetate
-
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
cephalosporin C
acetate + ?
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
corncob xylan + H2O
?
-
-
-
-
?
D-galactose pentaacetate + H2O
D-galactose + acetate
low activity
-
?
D-glucose penta-acetate + H2O
?
-
deacetylation
-
-
?
D-glucose penta-acetate + H2O
? + acetate
-
-
-
-
?
D-glucose pentaacetate + H2O
?
D-glucose pentaacetate + H2O
D-glucose + acetate
-
-
-
?
D-xylose tetraacetate + H2O
?
feruloylated (3-O-Ara)2-O-acetyl-Xyl in xylooligomer + H2O
feruloylated (3-O-Ara)-Xyl in xylooligomer + acetate
-
-
-
-
?
glucose pentaacetate
acetate + ?
glucose pentaacetate + H2O
?
-
-
-
?
glucose pentaacetate + H2O
glucose + acetate
-
-
?
glucose pentacetate + H2O
?
-
-
-
-
?
hardwood acetyl glucuronoxylan
?
insoluble oat spelt xylan
?
-
-
-
?
linalyl acetate + H2O
linalool + acetate
-
-
-
-
?
methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
methyl 2,3-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 2-O-acetyl-beta-D-xylopyranoside + methyl 3-O-acetyl-beta-D-xylopyranoside
methyl 2,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
methyl 3,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
methyl beta-cellobiose + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-cellobiose + vinyl acetate
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate + H2O
?
methyl beta-D-xylopyranoside 2,4-di-O-acetate + H2O
?
methyl beta-D-xylopyranoside 2-deoxy-2-fluoro-3,4-di-O-acetate + H2O
?
methyl beta-D-xylopyranoside 2-deoxy-3,4-di-O-acetate + H2O
?
methyl beta-D-xylopyranoside 3 deoxy-3-fluoro-2,4-di-O-acetate + H2O
?
methyl beta-D-xylopyranoside 3,4-di-O-acetate + H2O
?
methyl beta-D-xylopyranoside 3-deoxy-2,4-di-O-acetate + H2O
?
methyl beta-xylopyranoside + triacetin
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-xylopyranoside + vinyl acetate
?
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
N,N'-diacetylchitobiose + H2O
?
-
-
-
-
?
N,N'-diacetylchitobiose + H2O
chitobiose + acetate
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
p-nitrophenyl caprylate + H2O
p-nitrophenol + caprylate
p-nitrophenyl palmitate + H2O
p-nitrophenol + palmitate
penta-O-acetyl-D-glucose
acetate + ?
-
-
-
-
?
sucrose octaacetate + H2O
?
-
-
-
?
tertiary-butyl acetate + H2O
tert-butanol + acetate
-
-
-
-
?
tetraacetyl-xylopyranoside + H2O
? + acetate
-
-
-
?
tri-O-acetyl-D-galactal + H2O
D-galactal + acetate
-
-
?
tri-O-acetylglycerol
acetate + ?
-
-
-
-
?
triacetin
acetate + ?
-
-
-
-
?
xylan + acetate
xylan acetate
-
-
-
-
?
xylan + H2O
?
-
deacetylation of acetylated oat spelt xylan and DMSO-extracted beechwood xylan
-
-
?
xylan acetate + H2O
xylan + acetate
-
-
-
-
r
xylose tetraacetate
acetate + ?
xylose tetraacetate + H2O
?
xylose tetraacetate + H2O
xylose + acetate
additional information
?
-
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE2 and CE4
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE5
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE6
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE1
-
-
?
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE5
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
acetate + 4-nitrophenyl-beta-D-xylopyranoside
-
-
-
-
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
acetate + 4-nitrophenyl-beta-D-xylopyranoside
-
-
-
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
acetate + 4-nitrophenyl-beta-D-xylopyranoside
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
-
-
-
-
?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
DI175536
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
DI175536
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
7-aminocephalosporanic acid + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
?
-
-
-
-
?
Ac-MeGlcAXylan + H2O
?
a xylan polymer containing 4-O-methyl-D-glucopyranosyl uronic acid and acetyl groups
-
-
?
Ac-MeGlcAXylan + H2O
?
a xylan polymer containing 4-O-methyl-D-glucopyranosyl uronic acid and acetyl groups
-
-
?
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
-
5.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
-
5.8% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
-
5.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
-
5.8% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
acetylated birchwood xylan + H2O
deacetylated birchwood xylan + acetate
DI175536
-
-
-
?
acetylated birchwood xylan + H2O
deacetylated birchwood xylan + acetate
DI175536
-
-
-
?
acetylated chitosan + H2O
? + acetate
-
-
-
?
acetylated chitosan + H2O
? + acetate
-
-
-
?
acetylated colloidal chitin + H2O
? + acetate
-
-
-
?
acetylated colloidal chitin + H2O
? + acetate
-
-
-
?
acetylated glycol chitin + H2O
? + acetate
-
-
-
?
acetylated glycol chitin + H2O
? + acetate
-
-
-
?
acetylated xylan + H2O
?
from Eukalyptus wood
-
-
?
acetylated xylan + H2O
?
from Eukalyptus wood
-
-
?
acetylated xylan + H2O
partially deacetylated xylan + acetate
-
-
-
?
acetylated xylan + H2O
partially deacetylated xylan + acetate
-
-
-
?
acetylated xylan + H2O
xylan + acetate
-
-
-
?
acetylated xylan + H2O
xylan + acetate
acetylated xylan is the best substrate, half maximal activity with birchwood xylan
-
?
acetylated xylan + H2O
xylan + acetate
enzyme is a key component of xylan and cell wall degradation
-
?
acetylated xylan + H2O
xylan + acetate
-
substrate prepared from birchwood, the recombinant enzyme acts synergistically with xylanase to degrade acetylated xylan
-
?
acetylated xylan + H2O
xylan + acetate
the enzyme acts synergistically with xylanase to degrade acetylated xylan
-
?
acetylated xylan + H2O
xylan + acetate
-
-
-
-
?
acetylated xylan + H2O
xylan + acetate
-
-
?
acetylated xylan + H2O
xylan + acetate
acetate esters of D-xylanopyranose residues in xylan substrates
-
?
acetylated xylan + H2O
xylan + acetate
-
substrate from birchwood
-
?
acetylated xylan + H2O
xylan + acetate
-
-
-
?
acetylated xylan + H2O
xylan + acetate
catalytic mechanism, substrate with mono- and double acetylated residues, the enzyme cannot remove acetyl groups located close to large side groups such as 4-O-methylglucuronic acid
-
?
acetylated xylan + H2O
xylan + acetate
-
-
-
?
acetylated xylan + H2O
xylan + acetate
exhibits activity toward acetylated oat spelt xylan
-
-
?
acetylated xylan + H2O
xylan + acetate
exhibits activity toward acetylated oat spelt xylan
-
-
?
acetylated xylooligosaccharide + H2O
?
-
-
-
?
acetylated xylooligosaccharide + H2O
?
-
-
-
?
acetylated xylooligosaccharides + H2O
?
-
-
-
-
?
acetylated xylooligosaccharides + H2O
?
-
-
-
-
?
acetylxylan
acetate + ?
-
steamed birchwood xylan
-
-
?
acetylxylan
acetate + ?
-
-
-
-
?
acetylxylan
acetate + ?
-
-
-
-
?
acetylxylan
acetate + ?
-
birchwood acetylxylan
-
-
?
acetylxylan
acetate + ?
-
birchwood acetylxylan
-
-
?
acetylxylan
acetate + ?
-
-
-
-
?
acetylxylan
acetate + ?
-
-
-
-
?
acetylxylan
acetate + ?
-
-
-
?
acetylxylan
acetate + ?
-
larch xylan
-
-
?
acetylxylan
acetate + ?
-
larch xylan
-
-
?
acetylxylan
acetate + ?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylanacetate + ?
?
-
-
-
-
?
acetylxylanacetate + ?
?
-
-
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; 2,3-di-O acetylated Xylp were nearly completely deacetylated by CE5
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; 2,3-di-O acetylated Xylp were nearly completely deacetylated by CE1
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; 2,3-di-O acetylated Xylp were nearly completely deacetylated by CE5
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE3
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE16
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
alpha-naphthyl acetate
acetate + 2-naphthol
-
-
-
-
?
alpha-naphthyl acetate
acetate + 2-naphthol
-
-
-
-
?
alpha-naphthyl acetate
acetate + 2-naphthol
-
-
-
-
?
alpha-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
-
-
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
-
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
-
14.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
-
15.2% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
-
14.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
an acetic ester + H2O
an alcohol + acetate
-
-
-
-
?
an acetic ester + H2O
an alcohol + acetate
-
-
-
-
?
an acetic ester + H2O
an alcohol + acetate
-
-
-
?
an acetic ester + H2O
an alcohol + acetate
-
-
-
?
an acetic ester + H2O
an alcohol + acetate
-
-
-
?
aspen acetyl glucuronoxylan + H2O
?
-
-
-
-
?
aspen acetyl glucuronoxylan + H2O
?
-
-
-
-
?
aspen acetyl glucuronoxylan + H2O
?
-
-
-
-
?
aspen acetyl glucuronoxylan + H2O
?
-
-
-
-
?
aspen acetyl glucuronoxylan + H2O
?
-
-
-
-
?
beechwood acetyl glucuronoxylan + H2O
?
-
-
-
-
?
beechwood acetyl glucuronoxylan + H2O
?
-
-
-
-
?
beechwood xylan + H2O
?
-
-
-
?
beechwood xylan + H2O
?
-
-
-
?
beta-D-xylose tetraacetate + H2O
?
-
-
-
?
beta-D-xylose tetraacetate + H2O
?
-
-
-
?
beta-D-xylose tetraacetate + H2O
?
-
-
-
?
beta-naphthyl propionate + H2O
2-naphthol + propionate
-
41.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
beta-naphthyl propionate + H2O
2-naphthol + propionate
-
57.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
birchwood acetyl glucuronoxylan + H2O
?
-
-
-
-
?
birchwood acetyl glucuronoxylan + H2O
?
-
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
very low activity
-
?
cellulose acetate + H2O
cellulose + acetate
-
cleavage of acetyl at positions C2 and C3
-
?
cellulose acetate + H2O
cellulose + acetate
-
cleavage of acetyl at position C3
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
-
cleavage of acetyl at position C2
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
deacetylation
-
-
?
D-glucose pentaacetate + H2O
?
-
10.0% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
D-glucose pentaacetate + H2O
?
-
10.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
D-xylose tetraacetate + H2O
?
-
10.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A
-
-
?
D-xylose tetraacetate + H2O
?
-
5.0% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B
-
-
?
glucose pentaacetate
acetate + ?
-
glucose-beta-D-pentaacetate
-
-
?
glucose pentaacetate
acetate + ?
-
-
-
-
?
hardwood acetyl glucuronoxylan
?
-
no complete deacetylation, complete deacetylation of a hardwood acetyl glucuronoxylan requires additional deacetylating enzyme(s)
-
-
?
hardwood acetyl glucuronoxylan
?
-
the enzyme from Aspergillus niger is highly active compared to other acetylxylan esterases independently of the degree of polymerisation of the substrate. Mode of action, substrate specificity, and role of the family CE1 enzyme during deacetylation of soluble natural substrates, i.e. neutral and acidic O-acetylated xylooligosaccharides
-
-
?
hardwood acetyl glucuronoxylan
?
-
no complete deacetylation, complete deacetylation of a hardwood acetyl glucuronoxylan requires additional deacetylating enzyme(s)
-
-
?
hardwood acetyl glucuronoxylan
?
-
the target of the enzyme are 2- and 3-monoacetylated xylopyranosyl residues and also the 2,3-di-O-acetylated xylopyranosyl residues. The enzyme does not recognize as a substrate the 3-O-acetyl group on xylopyranosyl residues alpha-1,2-substituted with 4-O-methyl-D-glucuronic acid
-
-
?
hardwood acetyl glucuronoxylan
?
xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position
-
-
?
hardwood acetyl glucuronoxylan
?
xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position
-
-
?
hardwood acetyl glucuronoxylan
?
-
no complete deacetylation, complete deacetylation of a hardwood acetyl glucuronoxylan requires additional deacetylating enzyme(s)
-
-
?
hardwood acetyl glucuronoxylan
?
-
no complete deacetylation, complete deacetylation of a hardwood acetyl glucuronoxylan requires additional deacetylating enzyme(s)
-
-
?
hardwood acetyl glucuronoxylan
?
-
the target of the enzyme are 2- and 3-monoacetylated xylopyranosyl residues. The enzyme does not recognize as a substrate the 3-O-acetyl group on xylopyranosyl residues alpha-1,2-substituted with 4-O-methyl-D-glucuronic acid
-
-
?
hardwood acetyl glucuronoxylan
?
-
mode of action, substrate specifiicity, and role of the family CE1 enzyme during deacetylation of soluble natural substrates, i.e. neutral and acidic O-acetylated xylooligosaccharides
-
-
?
hardwood acetyl glucuronoxylan
?
-
no complete deacetylation, complete deacetylation of a hardwood acetyl glucuronoxylan requires additional deacetylating enzyme(s)
-
-
?
hardwood acetyl glucuronoxylan
?
-
the enzyme can act on di-O-acetylated Xylp residues, but may have different activities towards the O-2- and O-3-linked acetyl groups, since the content of monoacetylated Xylp units is high after enzyme action. Mode of action, substrate specifiicity, and role of the family CE5 enzyme during deacetylation of soluble natural substrates, i.e. neutral and acidic O-acetylated xylooligosac-charides, overview
-
-
?
methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
methyl 2,4-di-O-acetyl beta-D-xylopyranoside + methyl 2,3-di-O-acetyl beta-D-xylopyranoside + methyl 3,4-di-O-acetyl beta-D-xylopyranoside in the ratio 1.0:3.0:0.15
?
methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 2,3-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 2-O-acetyl-beta-D-xylopyranoside + methyl 3-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 2,3-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 2-O-acetyl-beta-D-xylopyranoside + methyl 3-O-acetyl-beta-D-xylopyranoside
-
-
-
-
?
methyl 2,3-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 2-O-acetyl-beta-D-xylopyranoside + methyl 3-O-acetyl-beta-D-xylopyranoside
-
-
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside + methyl 2-O-acetyl-beta-D-xylopyranoside
?
methyl 2,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
acetate + methyl 1-O-acetyl-beta-D-xylopyranoside + methyl 4-O-acetyl-beta-D-xylopyranoside
?
methyl 2,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 2,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 3,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
Acetate + methyl 3-O-acetyl-beta-D-xylopyranoside + methyl 4-O-acetyl-beta-D-xylopyranoside
?
methyl 3,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl 3,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
-
-
-
?
methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate + H2O
?
-
-
-
-
?
methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate + H2O
?
-
-
-
-
?
methyl beta-D-xylopyranoside 2,4-di-O-acetate + H2O
?
-
43% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 2,4-di-O-acetate + H2O
?
-
500% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 2-deoxy-2-fluoro-3,4-di-O-acetate + H2O
?
-
180% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 2-deoxy-2-fluoro-3,4-di-O-acetate + H2O
?
-
220% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 2-deoxy-3,4-di-O-acetate + H2O
?
-
142% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 2-deoxy-3,4-di-O-acetate + H2O
?
-
38% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3 deoxy-3-fluoro-2,4-di-O-acetate + H2O
?
-
186% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3 deoxy-3-fluoro-2,4-di-O-acetate + H2O
?
-
650% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3,4-di-O-acetate + H2O
?
-
322% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3,4-di-O-acetate + H2O
?
-
170% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3-deoxy-2,4-di-O-acetate + H2O
?
-
8% of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
methyl beta-D-xylopyranoside 3-deoxy-2,4-di-O-acetate + H2O
?
-
12 of the activity with methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate
-
-
?
oat spelt xylan + H2O
?
-
-
-
?
oat spelt xylan + H2O
?
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
?
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
-
-
-
?
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
-
-
-
?
p-nitrophenyl caprylate + H2O
p-nitrophenol + caprylate
-
-
-
-
?
p-nitrophenyl caprylate + H2O
p-nitrophenol + caprylate
-
-
-
-
?
p-nitrophenyl palmitate + H2O
p-nitrophenol + palmitate
-
-
-
-
?
p-nitrophenyl palmitate + H2O
p-nitrophenol + palmitate
-
-
-
-
?
xanthan + H2O
?
-
bacterial polysaccharide xanthan is a polymer having a (1->4)-linked beta-D-glucopyranosyl backbone, with a beta-D-mannopyranosyl-(1->4)-beta-D-glucopyranosyluronic acid-(1->2)-alpha-D-mannopyranosyl side chain substituted to every other glucopyranosyl residue at the O-3 position. Enzyme activity towards xanthan is only observed when xanthan molecules are in the disordered conformation in complete absence of salt. The enzyme specifically removes the acetyl groups positioned on the inner mannose, acetyl groups positioned on the outer mannose are not removed at all. After a prolonged incubation at optimal conditions, 57% of all acetyl groups, representing 70% of all acetyl groups on the inner mannose units, are hydrolyzed
-
-
?
xanthan + H2O
?
-
bacterial polysaccharide xanthan is a polymer having a (1->4)-linked beta-D-glucopyranosyl backbone, with a beta-D-mannopyranosyl-(1->4)-beta-D-glucopyranosyluronic acid-(1->2)-alpha-D-mannopyranosyl side chain substituted to every other glucopyranosyl residue at the O-3 position. Enzyme activity towards xanthan is only observed when xanthan molecules are in the disordered conformation in complete absence of salt. The enzyme specifically removes the acetyl groups positioned on the inner mannose, acetyl groups positioned on the outer mannose are not removed at all. After a prolonged incubation at optimal conditions, 57% of all acetyl groups, representing 70% of all acetyl groups on the inner mannose units, are hydrolyzed
-
-
?
xylose tetraacetate
acetate + ?
-
-
-
-
?
xylose tetraacetate
acetate + ?
-
-
-
-
?
xylose tetraacetate + H2O
?
-
-
-
?
xylose tetraacetate + H2O
?
-
-
-
?
xylose tetraacetate + H2O
xylose + acetate
-
-
-
?
xylose tetraacetate + H2O
xylose + acetate
-
-
?
additional information
?
-
-
inability of CE4 AcXEs to catalyze deacetylation of 2,3-di-O-acetylated xylopyranosyl residues. Substrate specificity together with endo-beta-1,4-xylanase of GH10 family from Clostridium thermocellum, mode of action of the CE4 family enzyme, overview. Regardless of the polymerization degree, each neutral oligosaccharide is converted into three forms: completely deacetylated and mono- and di-O-acetylated
-
-
?
additional information
?
-
-
isoform CE4 does not hydrolyze 4-nitrophenyl acetate
-
-
?
additional information
?
-
-
no activity towards acyl-chain substrates containing four or more carbon atoms, methyl esters of ferulic, caffeic or sinapic acids
-
-
?
additional information
?
-
-
no activity with acetylated apple pectin or acetylated sugar beet pectin
-
-
?
additional information
?
-
-
combined action of acetyl esterases and acetylxylan esterases enhances deacetylation activity
-
-
?
additional information
?
-
-
the enzyme shows partial activity with acetylated polysaccharides and neutral and acidic xylooligosaccharides, substrates are from Eucalyptus globulus xylan hydrolysate, and soluble extract of corn silage
-
-
?
additional information
?
-
no activity towards acyl-chain substrates containing four or more carbon atoms, methyl esters of ferulic, caffeic or sinapic acids
-
-
?
additional information
?
-
-
no activity towards acyl-chain substrates containing four or more carbon atoms, methyl esters of ferulic, caffeic or sinapic acids
-
-
?
additional information
?
-
-
induced by corncob powder and xylan
-
-
?
additional information
?
-
-
accessory enzyme in plant cell wall hemicellulose biodegradation pathway
-
-
?
additional information
?
-
Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview
-
-
?
additional information
?
-
-
Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview
-
-
?
additional information
?
-
Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview
-
-
?
additional information
?
-
-
accessory enzyme in plant cell wall hemicellulose biodegradation pathway
-
-
?
additional information
?
-
-
induced by corncob powder and xylan
-
-
?
additional information
?
-
-
the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41
-
-
?
additional information
?
-
-
the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments
-
-
?
additional information
?
-
-
the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41
-
-
?
additional information
?
-
-
the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments
-
-
?
additional information
?
-
the enzyme shows regioselective specific activity with acetylated saccharides
-
-
?
additional information
?
-
-
the enzyme shows regioselective specific activity with acetylated saccharides
-
-
?
additional information
?
-
the enzyme shows regioselective specific activity with acetylated saccharides
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe2 has a clear preference for acetylated xylo-oligosaccharides with a high degree of substitution. For Axe2 the size of the oligomer is irrelevant. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe2 has a clear preference for acetylated xylo-oligosaccharides with a high degree of substitution. For Axe2 the size of the oligomer is irrelevant. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe3 shows no preference for acetylated xylo-oligosaccharides with a high degree of substitution. Axe3 has a preference for large acetylated xylo-oligosaccharides when compared to smaller acetylated xylo-oligosaccharides. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe3 shows no preference for acetylated xylo-oligosaccharides with a high degree of substitution. Axe3 has a preference for large acetylated xylo-oligosaccharides when compared to smaller acetylated xylo-oligosaccharides. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 shows no activity with 4-nitrophenyl acetate, methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 shows no activity with 4-nitrophenyl acetate, methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 shows no activity with methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 shows no activity with methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe2 has a clear preference for acetylated xylo-oligosaccharides with a high degree of substitution. For Axe2 the size of the oligomer is irrelevant. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe2 has a clear preference for acetylated xylo-oligosaccharides with a high degree of substitution. For Axe2 the size of the oligomer is irrelevant. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 shows no activity with 4-nitrophenyl acetate, methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe2 shows no activity with 4-nitrophenyl acetate, methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe3 shows no preference for acetylated xylo-oligosaccharides with a high degree of substitution. Axe3 has a preference for large acetylated xylo-oligosaccharides when compared to smaller acetylated xylo-oligosaccharides. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 is able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Axe3 shows no preference for acetylated xylo-oligosaccharides with a high degree of substitution. Axe3 has a preference for large acetylated xylo-oligosaccharides when compared to smaller acetylated xylo-oligosaccharides. Even though there is difference in substrate affinity towards acetylated xylooligosaccharides from Eucalyptus wood, the final hydrolysis products are the same for Axe2 and Axe3: xylo-oligosaccharides containing one acetyl group located at the non-reducing xylose residue that remains, overview
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 shows no activity with methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
acetyl xylan esterase Axe3 shows no activity with methyl ferulate, methyl sinapate, and methyl caffeate
-
-
?
additional information
?
-
no tributyrin hydrolyzing activity
-
-
?
additional information
?
-
-
the enzyme releases xylose from insoluble material of pretreated destarched corn bran, overview
-
-
?
additional information
?
-
-
not active with carboxymethylcellulose, larchwood xylan, ferrulic acid-arabinose-xylose polymer, p-nitrophenyl-alpha-L-arabinofuranoside, longer-chain fatty acid esters
-
-
?
additional information
?
-
-
the enzyme may play a role in enhancing hemicellulose degradation
-
-
?
additional information
?
-
-
the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A
-
-
?
additional information
?
-
-
not active with carboxymethylcellulose, larchwood xylan, ferrulic acid-arabinose-xylose polymer, p-nitrophenyl-alpha-L-arabinofuranoside, longer-chain fatty acid esters
-
-
?
additional information
?
-
-
the enzyme may play a role in enhancing hemicellulose degradation
-
-
?
additional information
?
-
-
the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A
-
-
?
additional information
?
-
wild-type protein does not show any binding activity to Avicel. The enzyme is a modular protein comprised of an esterase domain, a carbohydrate-binding module, and a region of unknown function (Fibrobacter succinogenes-specific paralogous module 1, FPm-1). The N-terminal half of Axe6B harbors the activity that cleaves side chain acetyl groups from xylan-like substrates, and the binding of insoluble xylan is determined to originate from FPm-1. Esterase activity is derived from a tetrad composed of Ser44, His273, Glu194, and Asp270, with both Glu194 and Asp270 functioning as helper acids, instead of a single carboxylate residue proposed to initiate catalysis
-
-
?
additional information
?
-
-
wild-type protein does not show any binding activity to Avicel. The enzyme is a modular protein comprised of an esterase domain, a carbohydrate-binding module, and a region of unknown function (Fibrobacter succinogenes-specific paralogous module 1, FPm-1). The N-terminal half of Axe6B harbors the activity that cleaves side chain acetyl groups from xylan-like substrates, and the binding of insoluble xylan is determined to originate from FPm-1. Esterase activity is derived from a tetrad composed of Ser44, His273, Glu194, and Asp270, with both Glu194 and Asp270 functioning as helper acids, instead of a single carboxylate residue proposed to initiate catalysis
-
-
?
additional information
?
-
-
no activity on alkyl esters longer than butanoate
-
-
?
additional information
?
-
the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan
-
-
?
additional information
?
-
-
the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan
-
-
?
additional information
?
-
the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan
-
-
?
additional information
?
-
-
1H-NMR spectroscopy analysis of positional and substrate specificity, positional specificity of the enzyme, overview
-
-
?
additional information
?
-
-
substrate specificity together with endo-beta-1,4-xylanase of GH10 family from Clostridium thermocellum, mode of action of the CE6 family enzyme, overview. On aspen glucuronoxylan hydrolysate, all neutral and acidic xylooligosaccharides present in the starting mixture are only partially deacetylated and persist in the reactionmixture mainly as mono- and di-O-acetates. The degree of acetylation is higher with shorter than longer oligosaccharides
-
-
?
additional information
?
-
-
no substrates: cellulose acetate, N-acetyl glycosamine. The enzyme shows activity towards glyceryl tributanoate and glyceryl trioleate, olive oil and fish oil
-
-
?
additional information
?
-
no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate
-
-
?
additional information
?
-
-
no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate
-
-
?
additional information
?
-
no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate
-
-
?
additional information
?
-
the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan
-
-
?
additional information
?
-
-
the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan
-
-
?
additional information
?
-
enzyme also exhibits a xylanase activity at an optimum pH and temperature of 5.0 and 80°C, respectively
-
-
?
additional information
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the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan
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induced with triacetin
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formation of monoacetates 4-, 3-, and 2-O-acetyl-methyl-beta-xylosepyranoside in a ratio 1:0.9:0.6, also of diacetates 2,3-, 3,4-, and 2,4-di-O-acetyl-methyl-beta-xylosepyranoside in a ratio 1:0.47:0.28
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substrate specificity together with endo-beta-1,4-xylanase of GH10 family from Clostridium thermocellum, overview. The enzyme converts acidic oligosaccharides mainly to mono- and di-O-acetyl derivatives, largest amount detected is mono-O-acetate is GlcAXyl8Ac, completely deacetylated MeGlcAXyl3, MeGlcAXyl4 and MeGlcAXyl5 are also observed, mode of action of the CE1 family enzyme, overview
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the enzyme is able to deacetylate chitinous substrate
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1H-NMR spectroscopy analysis of positional and substrate specificity, positional specificity of the enzyme, overview
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inability of CE4 AcXEs to catalyze deacetylation of 2,3-di-O-acetylated xylopyranosyl residues. Substrate specificity together with endo-beta-1,4-xylanase of GH10 family from Clostridium thermocellum, mode of action of the CE4 family enzyme, overview. Regardless of the polymerization degree, each neutral oligosaccharide is converted into three forms: completely deacetylated and mono- and di-O-acetylated
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it is proposed that AXE I and II act in succession in xylan degradation, first, xylan is attacked by AXE I and other xylanases possessing CBMs (which facilitate binding to lignocellulose), followed by other enzymes acting mainly on soluble substrates
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it is proposed that AXE I and II act in succession in xylan degradation, first, xylan is attacked by AXE I and other xylanases possessing CBMs (which facilitate binding to lignocellulose), followed by other enzymes acting mainly on soluble substrates
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alpha-naphthyl acetate is a poor substrate
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enzyme is involved in xylan degradation
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the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources
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determination of xylanase activity by measuring the release of reducing sugars from oat-spelt xylan, production of xylobiose, xylotriose and xylotetrose by a crude enzyme extract. The purified recombinant His-tagged acetylxylan esterase has broad substrate specificity for various carboxyl esters. Among the polyoxyethylenesorbate esters tested, the enzyme has the highest activity for polyoxyethylenesorbate monolaurate, but moderate or little activity for longer acyl chains. The enzyme is able to hydrolyze both acetylxylan and acetylcellulose, but acetylxylan has a better hydrolytic rate
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determination of xylanase activity by measuring the release of reducing sugars from oat-spelt xylan, production of xylobiose, xylotriose and xylotetrose by a crude enzyme extract. The purified recombinant His-tagged acetylxylan esterase has broad substrate specificity for various carboxyl esters. Among the polyoxyethylenesorbate esters tested, the enzyme has the highest activity for polyoxyethylenesorbate monolaurate, but moderate or little activity for longer acyl chains. The enzyme is able to hydrolyze both acetylxylan and acetylcellulose, but acetylxylan has a better hydrolytic rate
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the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources
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combined action of acetyl esterases and acetylxylan esterases enhances deacetylation activity
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the enzyme shows activity with acetylated polysaccharides and neutral and acidic xylooligosaccharides and broad specificity. Substrates are from Eucalyptus globulus xylan hydrolysate, and soluble extract of corn silage
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the enzyme prefers polymeric substrates
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the enzyme may play a role in enhancing hemicellulose degradation
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substrate specificity together with endo-beta-1,4-xylanase of GH10 family from Clostridium thermocellum, mode of action of the CE5 family enzyme, overview. After incubation with the esterase, free and monoacetylated Xyl2, Xyl3, and Xyl4 are detected, with the free oligosaccharides predominating. The complex mixture of acetylated aldouronic acids is converted to a mixture of short free and monoacetylated MeGlcAXylx species (x = 26). The mono-acetylated species are clearly more abundant than the corresponding non-acetylated species
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the enzyme acts independently of the degree of polymerisation of the substrate. Combined action of acetyl esterases and acetylxylan esterases enhances deacetylation activity
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alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate
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alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate
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exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate
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exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate
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exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate
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alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate
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