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Literature summary for 3.1.1.72 extracted from
- An Aspergillus oryzae acetyl xylan esterase: molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris (2006), J. Biotechnol., 121, 381-389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris, recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q75P26 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme appears to possess ASn-linked high-mannose oligosaccharides | Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Aspergillus oryzae | 1-naphthol + acetate | - |
? |  |
| acetylated birchwood xylan + H2O | - |
Aspergillus oryzae | acetate + birchwood xylan | - |
? | |
| alpha-naphthyl propionate + H2O | - |
Aspergillus oryzae | 1-naphthol + propionate | - |
? | |
| additional information | no activity towards acyl-chain substrates containing four or more carbon atoms, methyl esters of ferulic, caffeic or sinapic acids | Aspergillus oryzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AoAXE | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity | Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
room temperature, 1 h, about 50% loss of activity | Aspergillus oryzae |
| 6 | 7 | room temperature, 1 h, stable | Aspergillus oryzae |
| 8 | - |
room temperature, 1 h, about 10% loss of activity | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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