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Literature summary for 3.1.1.72 extracted from

  • Huang, Y.C.; Chen, Y.F.; Chen, C.Y.; Chen, W.L.; Ciou, Y.P.; Liu, W.H.; Yang, C.H.
    Production of ferulic acid from lignocellulolytic agricultural biomass by Thermobifida fusca thermostable esterase produced in Yarrowia lipolytica transformant (2011), Biores. Technol., 102, 8117-8122.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged extracellular enzyme in Yarrowia lipolytica strain P01g Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermobifida fusca
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermobifida fusca the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources ?
-
?
additional information Thermobifida fusca NTU22 the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources ?
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
gene axe
-
Thermobifida fusca NTU22
-
gene axe
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged extracellular enzyme 1.73fold from Yarrowia lipolytica strain P01g by nickel affinity chromatography Thermobifida fusca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
41.11-70.94 U/ml esterase activity in the fermentation broth of recombinant Yarrowia lipolytica strain P01g Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Thermobifida fusca 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Thermobifida fusca NTU22 4-nitrophenol + acetate
-
?
additional information the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources Thermobifida fusca ?
-
?
additional information the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources Thermobifida fusca NTU22 ?
-
?

Synonyms

Synonyms Comment Organism
acetyl xylan esterase
-
Thermobifida fusca
AXE
-
Thermobifida fusca
More acetyl xylan esterase Axe belongs to the carbohydrate esterase family 11 Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified recombinant enzyme, about 70% of the original esterase activity remains after heat treatment for 3 h Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermobifida fusca

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 purified recombinant enzyme, stable at 4°C for 24 h Thermobifida fusca