Cloned (Comment) | Organism |
---|---|
expression of the His-tagged extracellular enzyme in Yarrowia lipolytica strain P01g | Thermobifida fusca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermobifida fusca | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermobifida fusca | the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources | ? | - |
? | |
additional information | Thermobifida fusca NTU22 | the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
gene axe | - |
Thermobifida fusca NTU22 | - |
gene axe | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged extracellular enzyme 1.73fold from Yarrowia lipolytica strain P01g by nickel affinity chromatography | Thermobifida fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
41.11-70.94 U/ml esterase activity in the fermentation broth of recombinant Yarrowia lipolytica strain P01g | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Thermobifida fusca | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Thermobifida fusca NTU22 | 4-nitrophenol + acetate | - |
? | |
additional information | the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources | Thermobifida fusca | ? | - |
? | |
additional information | the enzyme performs production of ferulic acid from lignocellulolytic agricultural biomass. Rice bran, wheat bran, bagasse and corncob are used as hydrolysis substrates for the esterase in combination with xylanase, with corncob giving the best ferulic acid yield, rice bran and bagasse are poor sources | Thermobifida fusca NTU22 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Thermobifida fusca |
AXE | - |
Thermobifida fusca |
More | acetyl xylan esterase Axe belongs to the carbohydrate esterase family 11 | Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, about 70% of the original esterase activity remains after heat treatment for 3 h | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermobifida fusca |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | purified recombinant enzyme, stable at 4°C for 24 h | Thermobifida fusca |