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Literature summary for 3.1.1.72 extracted from

  • Puchart, V.; Gariepy, M.C.; Shareck, F.; Dupont, C.
    Identification of catalytically important amino acid residues of Streptomyces lividans acetylxylan esterase A from carbohydrate esterase family 4 (2006), Biochim. Biophys. Acta, 1764, 263-274.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptomyces lividans

Protein Variants

Protein Variants Comment Organism
D12A no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 6.5% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.5% of the wild-type activity Streptomyces lividans
D12N activity with acetylated xylan is 3.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 8.1% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.3% of the wild-type activity Streptomyces lividans
D130A no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 11.2% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.5% of the wild-type activity Streptomyces lividans
D130E activity with acetylated xylan is 27.7% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 14.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.1% of the wild-type activity Streptomyces lividans
D130N no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 7.0% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.1% of the wild-type activity Streptomyces lividans
D130Q activity with acetylated xylan is 10% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 12.1% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.4% of the wild-type activity Streptomyces lividans
D13A no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 7.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 8.6% of the wild-type activity Streptomyces lividans
D156A activity with acetylated xylan is 42% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 41.4% of the wild-type activity, activity with N,N'-diacetylchitobiose is 48.6% of the wild-type activity Streptomyces lividans
D156E activity with acetylated xylan is 81.7% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 50.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 56.7% of the wild-type activity Streptomyces lividans
D156N activity with acetylated xylan is 52.5% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 35.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 24.2% of the wild-type activity Streptomyces lividans
D156Q activity with acetylated xylan is 63.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 36.6% of the wild-type activity, activity with N,N'-diacetylchitobiose is 19.6% of the wild-type activity Streptomyces lividans
H155A activity with acetylated xylan is 5.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 9.9% of the wild-type activity, activity with N,N'-diacetylchitobiose is 2.8% of the wild-type activity Streptomyces lividans
H62A activity with acetylated xylan is 13.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 10.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.2% of the wild-type activity Streptomyces lividans
H66A activity with acetylated xylan is 30.5% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 8.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 3.5% of the wild-type activity Streptomyces lividans
H68A activity with acetylated xylan is 94.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 36% of the wild-type activity, activity with N,N'-diacetylchitobiose is 35.1% of the wild-type activity Streptomyces lividans
S63A/H66A activity with acetylated xylan is 63.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 11.9% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.0% of the wild-type activity Streptomyces lividans
S63A/H66D activity with acetylated xylan is 32.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 5.5% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.4% of the wild-type activity Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4-tri-O-acetyl-beta-D-xylopyranoside + H2O
-
Streptomyces lividans ?
-
?
acetylated xylan + H2O
-
Streptomyces lividans xylan + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Streptomyces lividans ?
-
?

Synonyms

Synonyms Comment Organism
acetylxylan esterase A
-
Streptomyces lividans