Cloned (Comment) | Organism |
---|---|
- |
Streptomyces lividans |
Protein Variants | Comment | Organism |
---|---|---|
D12A | no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 6.5% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.5% of the wild-type activity | Streptomyces lividans |
D12N | activity with acetylated xylan is 3.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 8.1% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.3% of the wild-type activity | Streptomyces lividans |
D130A | no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 11.2% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.5% of the wild-type activity | Streptomyces lividans |
D130E | activity with acetylated xylan is 27.7% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 14.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 6.1% of the wild-type activity | Streptomyces lividans |
D130N | no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 7.0% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.1% of the wild-type activity | Streptomyces lividans |
D130Q | activity with acetylated xylan is 10% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 12.1% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.4% of the wild-type activity | Streptomyces lividans |
D13A | no activity with acetylated xylan, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 7.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 8.6% of the wild-type activity | Streptomyces lividans |
D156A | activity with acetylated xylan is 42% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 41.4% of the wild-type activity, activity with N,N'-diacetylchitobiose is 48.6% of the wild-type activity | Streptomyces lividans |
D156E | activity with acetylated xylan is 81.7% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 50.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 56.7% of the wild-type activity | Streptomyces lividans |
D156N | activity with acetylated xylan is 52.5% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 35.8% of the wild-type activity, activity with N,N'-diacetylchitobiose is 24.2% of the wild-type activity | Streptomyces lividans |
D156Q | activity with acetylated xylan is 63.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 36.6% of the wild-type activity, activity with N,N'-diacetylchitobiose is 19.6% of the wild-type activity | Streptomyces lividans |
H155A | activity with acetylated xylan is 5.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 9.9% of the wild-type activity, activity with N,N'-diacetylchitobiose is 2.8% of the wild-type activity | Streptomyces lividans |
H62A | activity with acetylated xylan is 13.2% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 10.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.2% of the wild-type activity | Streptomyces lividans |
H66A | activity with acetylated xylan is 30.5% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 8.3% of the wild-type activity, activity with N,N'-diacetylchitobiose is 3.5% of the wild-type activity | Streptomyces lividans |
H68A | activity with acetylated xylan is 94.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 36% of the wild-type activity, activity with N,N'-diacetylchitobiose is 35.1% of the wild-type activity | Streptomyces lividans |
S63A/H66A | activity with acetylated xylan is 63.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 11.9% of the wild-type activity, activity with N,N'-diacetylchitobiose is 5.0% of the wild-type activity | Streptomyces lividans |
S63A/H66D | activity with acetylated xylan is 32.9% of the wild-type activity, activity with methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is 5.5% of the wild-type activity, activity with N,N'-diacetylchitobiose is 4.4% of the wild-type activity | Streptomyces lividans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,4-tri-O-acetyl-beta-D-xylopyranoside + H2O | - |
Streptomyces lividans | ? | - |
? | |
acetylated xylan + H2O | - |
Streptomyces lividans | xylan + acetate | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Streptomyces lividans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetylxylan esterase A | - |
Streptomyces lividans |