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Literature summary for 3.1.1.72 extracted from

  • Martinez-Martinez, I.; Montoro-Garcia, S.; Lozada-Ramirez, J.D.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate (2007), Anal. Biochem., 369, 210-217.
    View publication on PubMed

Application

Application Comment Organism
analysis colorimetric method based on the color changes produced in a pH indicator occurring during the deacetylating reaction of 7-aminocephalosporanic acid, cephalosporin C, or acetylated xylan caused by acetyl xylan esterase from Bacillus pumilus CECT 5072 Bacillus pumilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta (DE3) Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
CECT 5072
-
Bacillus pumilus CECT 5072
-
CECT 5072
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminocephalosporanic acid + H2O
-
Bacillus pumilus ?
-
?
7-aminocephalosporanic acid + H2O
-
Bacillus pumilus CECT 5072 ?
-
?
acetylxylanacetate + ?
-
Bacillus pumilus ?
-
?
acetylxylanacetate + ?
-
Bacillus pumilus CECT 5072 ?
-
?
cephalosporin C + H2O
-
Bacillus pumilus deacetylcephalosporin C + acetate
-
?
cephalosporin C + H2O
-
Bacillus pumilus CECT 5072 deacetylcephalosporin C + acetate
-
?

Synonyms

Synonyms Comment Organism
AXE
-
Bacillus pumilus