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Literature summary for 3.1.1.72 extracted from

  • Mai-Gisondi, G.; Maaheimo, H.; Chong, S.; Hinz, S.; Tenkanen, M.; Master, E.
    Functional comparison of versatile carbohydrate esterases from families CE1, CE6 and CE16 on acetyl-4-O-methylglucuronoxylan and acetyl-galactoglucomannan (2017), Biochim. Biophys. Acta, 1861, 2398-2405 .
    View publication on PubMed
Search for more literature for 3.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information fusion of family 3 carbohydrate binding module CBM3 from Clostridium thermocellum to the carboxyl terminus of the acetyl xylan esterase. CBM3 fusion has negligible impact on the thermostability or regioselectivity of AXE, activities towards acetylated corncob xylan, 4-methylumbelliferyl acetate, 4-nitrophenyl acetate, and cellobiose octaacetate are also unchanged. The activity of the fusion protein on cellulose acetate is 4fold increased compared with wild-type Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
0.05
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
0.13
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
0.24
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Aspergillus nidulans 4-methylumbelliferone + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans 4-nitrophenol + acetate
-
 ?
cellobiose octaacetate + H2O
-
 Aspergillus nidulans ? + acetate
-
 ?
cellulose acetate + H2O
-
 Aspergillus nidulans ? + acetate
-
 ?
corncob xylan + H2O
-
 Aspergillus nidulans ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life more than 48 h Aspergillus nidulans
60
-
 half-life 38.6 min Aspergillus nidulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
59
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
96
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
163
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
696
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
726
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
1310
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
1370
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans