Cloned (Comment) | Organism |
---|---|
gene axe, functional expression of the enzyme with a C-terminal His-tag under the alcohol oxidase 1 promoter in Pichia pastoris strain KM71, the recombinant enzyme is secreted, subcloning in Escherichia coli strain TOP10 | Coprinopsis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | presence of two additional amino acid residues at the enzyme's native N-terminus help stabilize the enzyme against the protease cleavages without affecting the enzyme activity | Coprinopsis cinerea |
General Stability | Organism |
---|---|
presence of two additional amino acid residues at the enzyme's native N-terminus help stabilize the enzyme against the protease cleavages without affecting the enzyme activity | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl butyrate | pH 8.0, 40°C, recombinant enzyme | Coprinopsis cinerea | |
4.3 | - |
4-nitrophenyl acetate | pH 8.0, 40°C, recombinant enzyme | Coprinopsis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
39966 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
40500 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
65500 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated xylan + H2O | Coprinopsis cinerea | - |
partially deacetylated xylan + acetate | - |
? | |
acetylated xylan + H2O | Coprinopsis cinerea Okayama 7 | - |
partially deacetylated xylan + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | Q09IZ4 | - |
- |
Coprinopsis cinerea Okayama 7 | Q09IZ4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has two potential N-glycosylation site, presence of N-glycosylation on the recombinant enzyme with predominant N-glycans HexNAc2Hex9-16, deglycosylation by PNGase F | Coprinopsis cinerea |
Purification (Comment) | Organism |
---|---|
recombinant C-terminal His-tagged enzyme from Pichia pastoris strain KM71 by nickel affinity chromatography | Coprinopsis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Coprinopsis cinerea | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Coprinopsis cinerea Okayama 7 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Coprinopsis cinerea | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Coprinopsis cinerea Okayama 7 | 4-nitrophenol + butyrate | - |
? | |
acetylated xylan + H2O | - |
Coprinopsis cinerea | partially deacetylated xylan + acetate | - |
? | |
acetylated xylan + H2O | - |
Coprinopsis cinerea Okayama 7 | partially deacetylated xylan + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Coprinopsis cinerea |
AXE | - |
Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme, sharp drop in activity above the optimum at 40°C | Coprinopsis cinerea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | activity range, profile overview | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | activity range, profile overview | Coprinopsis cinerea |