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Literature summary for 3.1.1.72 extracted from
- Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii (2012), Appl. Environ. Microbiol., 78, 3759-3762.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene axe1, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Star DE3 | Rasamsonia emersonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrophenol | - |
Rasamsonia emersonii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
2-naphthyl acetate | pH 5.0, 60°C, recombinant enzyme | Rasamsonia emersonii | |
| 11 | - |
4-nitrophenyl acetate | pH 5.0, 60°C, recombinant enzyme | Rasamsonia emersonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Rasamsonia emersonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rasamsonia emersonii | E9M3D1 | gene axe1 | - |
| Rasamsonia emersonii IMI 392299 | E9M3D1 | gene axe1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21-Star DE3 cannot be purified by nickel affinity chromatography, ultrafiltration, hydrophobic interaction chromatography, and again ultrafiltration are used instead | Rasamsonia emersonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-naphthyl acetate + H2O | - |
Rasamsonia emersonii | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Rasamsonia emersonii IMI 392299 | 2-naphthol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Rasamsonia emersonii | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Rasamsonia emersonii IMI 392299 | 4-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Rasamsonia emersonii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Axe1 | - |
Rasamsonia emersonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant enzyme, pH 5.0, half-life is 40.8 min in presence of 4-nitrophenyl acetate and 53.3 min in presence of 2-naphthyl acetate | Rasamsonia emersonii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rasamsonia emersonii | isoelectric focusing | - |
3.94 |
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