3.1.1.72: acetylxylan esterase

This is an abbreviated version!
For detailed information about acetylxylan esterase, go to the full flat file.

Reaction

Synonyms

Abf62A-Axe6A, Acel_0180, Acetic ester hydrolase, acetyl (xylan) esterase, Acetyl esterase, acetyl xylan esterase, acetyl xylan esterase 1, acetyl xylan esterase 2, acetyl xylan esterase A, acetyl xylan esterase I, acetyl xylan esterase II, acetyl xylan esterase, putative, acetyl xylan esterase/cephalosporin acetyl hydrolase, acetyl xylo-oligosaccharide esterase, Acetylglucomannan esterase, Acetylnaphthylesterase, acetylxylan esterase, acetylxylan esterase A, Acetyte esterase, AcXE, AE, alkaline acetyl xylan esterase, An12 g05010, AoAXE, AXE, AXE I, AXE II, AXE/CAH, Axe1, Axe2, Axe3, Axe6A, Axe6B, AxeA, AXEII, bifunctional acetylxylan esterase/xylanase, BnaA, C-esterase, CA esterase, CE1, CE1 AcXE, CE2, CE3, CE4, CE4 AcXE, CE5, CE5 AcXE, CE6, CE6 AcXE, Chloroacetate esterase, Chloroesterase, Citrus acetylesterase, CtAxe, DFP58_4623, Est2A, Esterase, acetyl, Esterase, C-, Heroin esterase, More, N-Acetylphosphinothricin deacetylase, NaM1, Naphthal AS-D chloroacetate deacetylase, PcAxe2, SlCE4, Vvaxe1, xylanase, Xyn10B, XynA, XynS20E

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.72 acetylxylan esterase

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Results	in table
11	Activating Compound
771	AA Sequence
15	Application
2	CAS Registry Number
49	Cloned(Commentary)
13	Crystallization (Commentary)
2	Expression
40	General Information
3	General Stability
81	Inhibitors
23	kcat/KM [mM/s]
47	KM Value [mM]
16	Localization
24	Metals/Ions
53	Molecular Weight [Da]
43	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
206	Organism
57	PDB ID
69	pH Optimum
13	pH Range
23	pH Stability
22	pI Value
14	Posttranslational Modification
56	Protein Variants
42	Purification (Commentary)
17	Reaction
17	Reaction Type
173	Reference
19	Source Tissue
118	Specific Activity [micromol/min/mg]
1	Storage Stability
455	Substrates and Products (Substrate)
82	Subunits
218	Synonyms
1	Systematic Name
68	Temperature Optimum [°C]
9	Temperature Range [°C]
52	Temperature Stability [°C]
32	Turnover Number [1/s]

KM Value

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KM Value on EC 3.1.1.72 - acetylxylan esterase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.43 - 2

(25R)-3beta-hydroxycholest-5-en-27-oate

3 entries

1.54

1-naphthyl acetate

Bacillus pumilus

-

-

23597

0.2 - 7

2-naphthyl acetate

4 entries

1

2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside

Streptomyces lividans

-

-

665703

0.079 - 0.8

4-methylumbelliferyl acetate

10 entries

0.04 - 11

4-nitrophenyl acetate

13 entries

0.11

4-nitrophenyl butyrate

Coprinopsis cinerea

Q09IZ4

pH 8.0, 40°C, recombinant enzyme

731027

0.3077 - 0.56

4-yethylumbelliferyl acetate

3 entries

0.056 - 2.66

Alpha-naphthyl acetate

3 entries

16.72

birchwood xylan

Neocallimastix patriciarum

B8YG19

pH 8.2, 58°C

713864

-

0.00186

p-nitrophenyl acetate

Thermobifida fusca

-

30°C, pH 8.0

697655

0.07 - 7

tetraacetyl-xylopyranoside

5 entries

additional information

additional information

2 entries

-

1.43

(25R)-3beta-hydroxycholest-5-en-27-oate

Aspergillus awamori

-

substrate: alpha-naphthylacetate, wild-type enzyme

664203

1.5 - 2

(25R)-3beta-hydroxycholest-5-en-27-oate

Aspergillus awamori

-

substrate: alpha-naphthylacetate, mutant enzyme D168A

664203

1.8

(25R)-3beta-hydroxycholest-5-en-27-oate

Aspergillus awamori

-

substrate: alpha-naphthylacetate, mutant enzyme S146A

664203

0.2

2-naphthyl acetate

uncultured microorganism

-

wild-type, pH 8.0, 25°C

750449

0.41

2-naphthyl acetate

uncultured microorganism

-

mutant N96S/F210L, pH 8.0, 25°C

750449

7

2-naphthyl acetate

Rasamsonia emersonii

E9M3D1

pH not specified in the publication, temperature not specified in the publication

741674

7

2-naphthyl acetate

Rasamsonia emersonii

E9M3D1

pH 5.0, 60°C, recombinant enzyme

729027

0.079

4-methylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C

750189

0.087

4-methylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

wild-type lacking CBM1, 50°C, pH 8.0

750189

0.087

4-methylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

wild-type, 50°C, pH 8.0

750189

0.094

4-methylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C

750189

0.099

4-methylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C

750189

0.13

4-methylumbelliferyl acetate

uncultured microorganism

-

wild-type, pH 8.0, 25°C

750449

0.13

4-methylumbelliferyl acetate

Aspergillus nidulans

-

wild-type, pH 7.5, 50°C

749963

0.24

4-methylumbelliferyl acetate

Aspergillus nidulans

-

fusion protein with CBM3, pH 7.5, 50°C

749963

0.47

4-methylumbelliferyl acetate

uncultured microorganism

-

mutant N96S/F210L, pH 8.0, 25°C

750449

0.8

4-methylumbelliferyl acetate

Flavobacterium johnsoniae

-

pH 7.0, 30°C

750138

0.04

4-nitrophenyl acetate

Aspergillus nidulans

-

fusion protein with CBM3, pH 7.5, 50°C

749963

0.05

4-nitrophenyl acetate

Aspergillus nidulans

-

wild-type, pH 7.5, 50°C

749963

0.092

4-nitrophenyl acetate

Phanerodontia chrysosporium

H2ESB9

pH 7.0, 30°C

742926

0.1

4-nitrophenyl acetate

uncultured microorganism

-

wild-type, pH 8.0, 25°C

750449

0.36

4-nitrophenyl acetate

Termitomyces clypeatus

-

pH 6.5, 50°C

650705

0.43

4-nitrophenyl acetate

Anoxybacillus flavithermus

-

pH 7.5, 50°C

752117

0.465

4-nitrophenyl acetate

Penicillium chrysogenum

A0A2H4V677

pH 7.0, 40'°C

751478

0.76

4-nitrophenyl acetate

uncultured microorganism

-

mutant N96S/F210L, pH 8.0, 25°C

750449

0.9

4-nitrophenyl acetate

Flavobacterium johnsoniae

-

pH 7.0, 30°C

750138

0.9

4-nitrophenyl acetate

Orpinomyces sp.

O59869

pH 6.7, 37°C

35242

4.3

4-nitrophenyl acetate

Coprinopsis cinerea

Q09IZ4

pH 8.0, 40°C, recombinant enzyme

731027

11

4-nitrophenyl acetate

Rasamsonia emersonii

E9M3D1

pH not specified in the publication, temperature not specified in the publication

741674

11

4-nitrophenyl acetate

Rasamsonia emersonii

E9M3D1

pH 5.0, 60°C, recombinant enzyme

729027

0.3077

4-yethylumbelliferyl acetate

Volvariella volvacea

Q09IZ4

pH 7.5, 50°C

679915

0.45

4-yethylumbelliferyl acetate

Thermoanaerobacterium saccharolyticum

-

isoenzyme I

23599

0.56

4-yethylumbelliferyl acetate

Thermoanaerobacterium saccharolyticum

-

isoenzyme II

23599

0.056

Alpha-naphthyl acetate

Fibrobacter succinogenes

-

pH 6.5, 37°C, acetylxylan esterase Axe6B

664492

0.084

Alpha-naphthyl acetate

Fibrobacter succinogenes

-

pH 6.5, 37°C, acetylxylan esterase Axe6A

664492

2.66

Alpha-naphthyl acetate

Fibrobacter succinogenes

-

-

23600

0.07

tetraacetyl-xylopyranoside

Fibrobacter succinogenes subsp. succinogenes S85

Q9F106

mutant TM2, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2

708940

0.08

tetraacetyl-xylopyranoside

Fibrobacter succinogenes subsp. succinogenes S85

Q9F106

wild-type, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2

708940

0.09

tetraacetyl-xylopyranoside

Fibrobacter succinogenes subsp. succinogenes S85

Q9F106

mutant TM1, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2

708940

0.2

tetraacetyl-xylopyranoside

Fibrobacter succinogenes subsp. succinogenes S85

Q9F106

mutants E194A, D270N and D270A, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2

708940

7

tetraacetyl-xylopyranoside

Fibrobacter succinogenes subsp. succinogenes S85

Q9F106

mutant E194N, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2

708940

additional information

additional information

Volvariella volvacea

B8YIE0

1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min

708516

-

additional information

additional information

Volvariella volvacea

-

1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min

708516

-