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0.0000045
-
purified enzyme, substrate 2,3,4-tri-O-acetyl beta-D-xylopyranoside
0.000056
-
purified enzyme, substrate 2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside
0.000063
-
purified enzyme, substrate 3-deoxy-2,4-di-O-acetyl beta-D-xylopyranoside
0.00016
-
purified enzyme, substrate 2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside
0.0013
-
substrate xanthan, pH 5.6, 40°C
0.0016
-
purified enzyme, substrate 3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside
0.02
mutant Y39P/W160S, substrate 1-naphthyl octanoate, pH 7.0, 37°C
0.03
mutant Y39G, substrate 1-naphthyl octanoate, pH 7.0, 37°C
0.034
-
purified enzyme, substrate 3,4-di-O-acetyl beta-D-xylopyranoside
0.04
mutant Y39P/W160S, substrate 1-naphthyl butanoate, pH 7.0, 37°C
0.05
mutant W160S, substrate 1-naphthyl octanoate, pH 7.0, 37°C
0.07
mutant W160A, substrate 1-naphthyl octanoate, pH 7.0, 37°C
0.08
purified recombinant enzyme, tri-O-acetyl-D-galactal
0.175
-
purified enzyme, substrate 2,4-di-O-acetyl beta-D-xylopyranoside
0.2
purified recombinant enzyme, xylose tetraacetate
0.84
mutant W160A, substrate 1-naphthyl butanoate, pH 7.0, 37°C
0.85
mutant W160S, substrate 1-naphthyl butanoate, pH 7.0, 37°C
0.87
mutant Y39G, substrate 1-naphthyl propionate, pH 7.0, 37°C
0.88
mutant Y39G/W160P, substrate 1-naphthyl propionate, pH 7.0, 37°C
1
mutant W160S, substrate 1-naphthyl acetate, pH 7.0, 37°C
1.06
-
with substrate 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
1.4
substrate 4-nitrophenyl acetate, pH not specified in the publication, temperature not specified in the publication
1.8
mutant Y39G/W160P, substrate 1-naphthyl acetate, pH 7.0, 37°C
100.1
DI175536
substrate 4-nitrophenyl acetyte, pH 8.0, 430°C
1086
-
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-methylumbelliferyl acetate
109100
-
purified recombinant His-tagged enzyme, pH 7.5, 60°C
113
-
acetylxylan esterase Axe6A
121.5
purified recombinant enzyme, substrate acetylated xylan
1228
acetylxylan esterase activity on beta-D-xylose tetraacetate, pH 8.2, 58°C, purified recombinant enzyme
1245
-
purified recombinant enzyme, pH 7.0, 40°C, substrate glucose pentacetate
14.7
-
substrate: alpha-naphthylacetate, mutant enzyme S146A
19
-
substrate: alpha-naphthylacetate, mutant enzyme D168A
19.7
-
substrate: alpha-naphthylacetate, wild-type enzyme
2.4
-
with substrate 4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
2.72
-
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl butyrate
21.9
wild-type, substrate 1-naphthyl acetate, pH 7.0, 37°C
2614
acetylxylan esterase activity on acetylxylan, pH 8.2, 58°C, purified recombinant enzyme
273.7
acetylxylan esterase activity on birchwood xylan, pH 8.2, 58°C, purified recombinant enzyme
28
substrate 2-naphthyl acetate, pH not specified in the publication, temperature not specified in the publication
2949
-
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl acetate
3.9
-
substrate: alpha-naphthylpropionate, mutant enzyme S146A
31.6
-
with substrate 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
4.8
substrate 4-nitrophenyl acetate, pH 7.0, 40°C
5
-
substrate: alpha-naphthylpropionate, wild-type D168A
55.3
-
with substrate 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
557.9
acetylxylan esterase activity on oat spelt xylan, pH 8.2, 58°C, purified recombinant enzyme
58.6
-
acetylxylan esterase Axe6B
580.3
acetylxylan esterase activity on 4-methylumbelliferyl acetate, pH 8.2, 58°C, purified recombinant enzyme
6.6
-
substrate: alpha-naphthylpropionate, wild-type enzyme
741
-
purified recombinant enzyme, pH 7.0, 40°C, substrate alpha-naphthyl acetate
8.3
-
substrate acetylated xylooligosaccharides, pH 5.6, 40°C
80
-
purified single enzyme
873.1
acetylxylan esterase activity on beechwood xylan, pH 8.2, 58°C, purified recombinant enzyme
9.3
wild-type, substrate 1-naphthyl propionate, pH 7.0, 37°C
962
-
purified recombinant enzyme, pH 7.0, 40°C, substrate beta-naphthyl acetate
0.1
-
below, with substrate 4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.1
-
below, with substrate 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
0.1
-
below, with substrate 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.1
-
below, with substrate 4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.1
-
below, with substrate 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
0.3
-
with substrate 4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.3
-
with substrate 4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.3
-
with substrate 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
0.31
mutant W160P, substrate 1-naphthyl butanoate, pH 7.0, 37°C
0.31
purified recombinant enzyme, glucose pentaacetate
0.49
mutant Y39P/W160S, substrate 1-naphthyl acetate, pH 7.0, 37°C
0.49
purified recombinant enzyme, 4-nitrophenyl acetate
0.5
-
with substrate 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
0.5
-
with substrate 4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
0.59
mutant Y39G/W160P, substrate 1-naphthyl butanoate, pH 7.0, 37°C
0.59
mutant Y39P/W160S, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.1
mutant W160A, substrate 1-naphthyl acetate, pH 7.0, 37°C
1.1
mutant W160P, substrate 1-naphthyl acetate, pH 7.0, 37°C
1.5
mutant W160S, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.5
mutant Y39P, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.6
mutant W160A, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.6
mutant Y39A, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.7
mutant W160P, substrate 1-naphthyl propionate, pH 7.0, 37°C
1.7
-
with substrate 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
1.7
-
with substrate 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
2.7
mutant Y39G, substrate 1-naphthyl acetate, pH 7.0, 37°C
2.7
-
with substrate 4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
2.7
-
with substrate 4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
3.06
-
with substrate 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
3.06
-
with substrate 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside, pH 5.5, 40°C
3.4
-
with substrate 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
3.4
-
with substrate 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
36.2
-
with substrate 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
36.2
-
with substrate 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside, pH 5.5, 40°C
39.86
pH 7.0, 30°C
39.86
purified recombinant enzyme, pH 7.0, 30°C
5.1
mutant Y39A, substrate 1-naphthyl acetate, pH 7.0, 37°C
5.1
mutant Y39P, substrate 1-naphthyl acetate, pH 7.0, 37°C
additional information
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additional information
-
-
additional information
-
-
additional information
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beta-xylosidase-coupled assay of acetylxylan esterases using monoacetylated 4-nitrophenyl beta-D-xylopyranosides
additional information
-
-
additional information
-
beta-xylosidase-coupled assay of acetylxylan esterases using monoacetylated 4-nitrophenyl beta-D-xylopyranosides
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
41.11-70.94 U/ml esterase activity in the fermentation broth of recombinant Yarrowia lipolytica strain P01g
additional information
-
-
additional information
-
-
additional information
in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min: 3448 IU/micromol with acetylated xylan as substrate, 830.9 IU/micromol with glycol chitin as substrate, 306.7 IU/micromol with chitosan as substrate, 361.1 IU/micromol with colloidal chitin as substrate
additional information
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in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min: 3448 IU/micromol with acetylated xylan as substrate, 830.9 IU/micromol with glycol chitin as substrate, 306.7 IU/micromol with chitosan as substrate, 361.1 IU/micromol with colloidal chitin as substrate
additional information
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23373 U/l/mol and 22692 U/l/mol are obtained for the wild-type and endo-H-treated enzyme forms, and 23306 U/l/mol, 23026 U/l/mol, and 22605 U/l/mol are recorded for the enzyme obtained from yeast cultures supplemented with 0.002, 0.0055 and 0.010 mg/ml tunicamycin, respectively