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Literature summary for 3.1.1.72 extracted from
- A novel bifunctional acetyl xylan esterase/arabinofuranosidase from Penicillium chrysogenum P33 enhances enzymatic hydrolysis of lignocellulose (2017), Microb. Cell Fact., 16, 166 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme displays significant synergy with a xylanase, with a degree of synergy of 1.35 for the hydrolysis of delignified corn stover. Release of glucose is increased by 51% from delignified corn stover when 2 mg of a commercial cellulase is replaced by an equivalent amount of Axe | Penicillium chrysogenum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Penicillium chrysogenum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.465 | - |
4-nitrophenyl acetate | pH 7.0, 40'°C | Penicillium chrysogenum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | A0A2H4V677 | - |
- |
| Penicillium chrysogenum P33 | A0A2H4V677 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Penicillium chrysogenum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
substrate 4-nitrophenyl acetate, pH 7.0, 40°C | Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Penicillium chrysogenum P33 | 4-nitrophenol + acetate | - |
? | |
| additional information | no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate | Penicillium chrysogenum | ? | - |
? | |
| additional information | no substrates: 4-hydroxy-3-methoxycinnamic acid methyl ester or ethyl-4-hydroxy-3-methoxycinnamate | Penicillium chrysogenum P33 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31160, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | 1 h, no loss of activity | Penicillium chrysogenum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | sequence reveals a GH62 domain exhibiting alpha-L-arabinofuranosidase activity, in addition to the expected esterase domain | Penicillium chrysogenum |
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Release 2023.1 (January 2023)
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