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Literature summary for 3.1.1.72 extracted from
- Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation (2002), Appl. Environ. Microbiol., 68, 6399-6402.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as C-terminally His-tagged and N-terminally S-protein tagged enzyme | Clostridium cellulovorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cellulosome | enzyme is a key component of the cellulosome performing xylan and cell wall degradation | Clostridium cellulovorans | 43263 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57038 | - |
x * 57038, DNA sequence calculation | Clostridium cellulovorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylated xylan + H2O | Clostridium cellulovorans | enzyme is a key component of xylan and cell wall degradation | xylan + acetate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium cellulovorans | Q8GH59 | gene xynA, enzyme with a double specificity as xylanase and acetylxylan esterase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant tagged protein from Escherichia coli | Clostridium cellulovorans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Clostridium cellulovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 121.5 | - |
purified recombinant enzyme, substrate acetylated xylan | Clostridium cellulovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Clostridium cellulovorans | 4-nitrophenol + acetate | - |
? | |
| acetylated xylan + H2O | acetylated xylan is the best substrate, half maximal activity with birchwood xylan | Clostridium cellulovorans | xylan + acetate | - |
? | |
| acetylated xylan + H2O | enzyme is a key component of xylan and cell wall degradation | Clostridium cellulovorans | xylan + acetate | - |
? | |
| cellulose acetate + H2O | very low activity | Clostridium cellulovorans | cellulose + acetate | - |
? | |
| D-galactose pentaacetate + H2O | low activity | Clostridium cellulovorans | D-galactose + acetate | - |
? | |
| N,N'-diacetylchitobiose + H2O | - |
Clostridium cellulovorans | chitobiose + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57038, DNA sequence calculation | Clostridium cellulovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl xylan esterase | - |
Clostridium cellulovorans |
| xylanase | - |
Clostridium cellulovorans |
| XynA | - |
Clostridium cellulovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium cellulovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Clostridium cellulovorans |
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Release 2023.1 (January 2023)
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