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Literature summary for 3.1.1.72 extracted from

  • Komiya, D.; Hori, A.; Ishida, T.; Igarashi, K.; Samejima, M.; Koseki, T.; Fushinobu, S.
    Crystal structure and substrate specificity modification of acetyl xylan esterase from Aspergillus luchuensis (2017), Appl. Environ. Microbiol., 83, e01251 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus luchuensis

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic machinery includes a catalytic triad (Ser119, His259, and Asp202) and an oxyanion hole (Cys40 and Ser120). Near the catalytic triad, aromatic residues Tyr39 and Trp160 form small pockets at both sides Aspergillus luchuensis

Protein Variants

Protein Variants Comment Organism
W160P significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
W160S significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
Y39A significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
Y39G significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
Y39G/W160P significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
Y39P significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis
Y39P/W160S significant decrease in the activity for the acetyl substrate,increase in activity for longer chain substrates Aspergillus luchuensis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Aspergillus luchuensis Q92194
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 mutant Y39P/W160S, substrate 1-naphthyl octanoate, pH 7.0, 37°C Aspergillus luchuensis
0.03
-
 mutant Y39G, substrate 1-naphthyl octanoate, pH 7.0, 37°C Aspergillus luchuensis
0.04
-
 mutant Y39P/W160S, substrate 1-naphthyl butanoate, pH 7.0, 37°C Aspergillus luchuensis
0.05
-
 mutant W160S, substrate 1-naphthyl octanoate, pH 7.0, 37°C Aspergillus luchuensis
0.07
-
 mutant W160A, substrate 1-naphthyl octanoate, pH 7.0, 37°C Aspergillus luchuensis
0.31
-
 mutant W160P, substrate 1-naphthyl butanoate, pH 7.0, 37°C Aspergillus luchuensis
0.49
-
 mutant Y39P/W160S, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
0.59
-
 mutant Y39G/W160P, substrate 1-naphthyl butanoate, pH 7.0, 37°C Aspergillus luchuensis
0.59
-
 mutant Y39P/W160S, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
0.84
-
 mutant W160A, substrate 1-naphthyl butanoate, pH 7.0, 37°C Aspergillus luchuensis
0.85
-
 mutant W160S, substrate 1-naphthyl butanoate, pH 7.0, 37°C Aspergillus luchuensis
0.87
-
 mutant Y39G, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
0.88
-
 mutant Y39G/W160P, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1
-
 mutant W160S, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
1.1
-
 mutant W160A, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
1.1
-
 mutant W160P, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
1.5
-
 mutant W160S, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1.5
-
 mutant Y39P, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1.6
-
 mutant W160A, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1.6
-
 mutant Y39A, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1.7
-
 mutant W160P, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
1.8
-
 mutant Y39G/W160P, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
2.7
-
 mutant Y39G, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
5.1
-
 mutant Y39A, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
5.1
-
 mutant Y39P, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis
9.3
-
 wild-type, substrate 1-naphthyl propionate, pH 7.0, 37°C Aspergillus luchuensis
21.9
-
 wild-type, substrate 1-naphthyl acetate, pH 7.0, 37°C Aspergillus luchuensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Aspergillus luchuensis 1-naphthol + acetate
-
 ?
1-naphthyl butanoate + H2O no substrate for wild-type Aspergillus luchuensis 1-naphthol + butanoate
-
 ?
1-naphthyl octanoate + H2O no substrate for wild-type Aspergillus luchuensis 1-naphthol + octanoate
-
 ?
1-naphthyl propionate + H2O
-
 Aspergillus luchuensis 1-naphthol + propionate
-
 ?

Synonyms

Synonyms Comment Organism
AxeA
-
 Aspergillus luchuensis