Literature summary for 3.1.1.72 extracted from

Mukhopadhyay, A.; Hazra, P.P.; Sengupta, T.; Saha, R.; Nandi, R.; Sengupta, S.
Protein-protein interaction conferring stability to an extracellular acetyl (xylan) esterase produced by Termitomyces clypeatus (2003), Biotechnol. Prog., 19, 720-726.

Search for more literature for 3.1.1.72

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	enzyme is not affected by 2-mercaptoethanol and EDTA	Termitomyces clypeatus

General Stability

General Stability	Organism
the enzyme aggregated with cellobiase shows higher thermal stability, temperature optimum, and resistance to chemical denaturants than the isolated purified enzyme	Termitomyces clypeatus

Inhibitors

Inhibitors	Comment	Organism	Structure
guanidinium hydrochloride	3.5-4.0 M, inactivation	Termitomyces clypeatus
additional information	no inhibition by xylose and acetate, enzyme is not affected by 2-mercaptoethanol and EDTA	Termitomyces clypeatus
SDS	0.5% inhibit the single enzyme, but 5% do not affect the aggregated enzyme	Termitomyces clypeatus
Trypsin	single enzyme loses its activity completely after 30 min at 37°C, while the aggregated enzyme shows 75-80% remaining activity after 60 min incubation at 37°C	Termitomyces clypeatus
Urea	7.0 M, inactivation	Termitomyces clypeatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.36	-	4-nitrophenyl acetate	pH 6.5, 50°C	Termitomyces clypeatus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Termitomyces clypeatus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	about 25% activation	Termitomyces clypeatus
Mg2+	about 25% activation	Termitomyces clypeatus
additional information	enzyme is not affected by Ca2+, Cu2+, and Zn2+	Termitomyces clypeatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	the enzyme is aggregated with a cellobiase tetramer to form a 80 kDa protein complex	Termitomyces clypeatus
30000	-	gel filtration	Termitomyces clypeatus

Organism

Organism	UniProt	Comment	Textmining
Termitomyces clypeatus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	-	Termitomyces clypeatus

Purification (Commentary)

Purification (Comment)	Organism
purification of the enzyme as a single 30 kDa protein, 60fold, and aggregated with cellobiase to a 80 kDa protein complex	Termitomyces clypeatus

Reaction

Reaction	Comment	Organism	Reaction ID
acetylxylan + 3 H2O = 3 acetic acid + xylan	catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.	Termitomyces clypeatus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Termitomyces clypeatus
80	-	purified single enzyme	Termitomyces clypeatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Termitomyces clypeatus	4-nitrophenol + acetate	-	?
acetylated xylan + H2O	substrate from birchwood	Termitomyces clypeatus	xylan + acetate	-	?
additional information	alpha-naphthyl acetate is a poor substrate	Termitomyces clypeatus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 30000, SDS-PAGE	Termitomyces clypeatus

Synonyms

Synonyms	Comment	Organism
acetyl (xylan) esterase	-	Termitomyces clypeatus
Acetyl esterase	-	Termitomyces clypeatus
AE	-	Termitomyces clypeatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	the temperature optimum is increased by aggregation of the enzyme with cellobiase	Termitomyces clypeatus
45	-	single enzyme	Termitomyces clypeatus
55	-	aggregated enzyme	Termitomyces clypeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the enzyme aggregated with cellobiase shows higher thermal stability than the isolated purified enzyme	Termitomyces clypeatus
35	-	single enzyme	Termitomyces clypeatus
50	-	30 min, pH 6.5, single enzyme, complete loss of activity	Termitomyces clypeatus
70	-	aggregated enzyme	Termitomyces clypeatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Termitomyces clypeatus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Termitomyces clypeatus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Termitomyces clypeatus	chromatocfocusing	7.4	7.3

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Release 2023.1 (January 2023)