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Literature summary for 3.1.1.72 extracted from

  • Liu, X.; Ding, S.
    Molecular characterization of a new acetyl xylan esterase (AXEII) from edible straw mushroom Volvariella volvacea with both de-O-acetylation and de-N-acetylation activity (2009), FEMS Microbiol. Lett., 295, 50-56.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA or 5'-end DNA fragment subcloned into the pGEM-T vector. Full-length gene encoding the mature esterase fused with a 6-histidine tag attached at the C-terminal and ligated at the EcoR1/Xba1 sites of the Picchia pastoris expression vector pPICZaA to yield the construct pPICZaA-AXEII. Transformed and expressed in Pichia pastoris Volvariella volvacea

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibits at higher (10 mM) concentrations Volvariella volvacea
Cu2+ 1 mM decreases enzyme activity by 12% Volvariella volvacea
EDTA concentrations greater than 1 mM almost eliminate the activity, but simultaneous addition of Co2+ (1 mM) and Mg2+ (1 mM) restores the activity Volvariella volvacea
Fe2+ 1 mM decreases enzyme activity by 59% Volvariella volvacea
Mn2+ 1 mM decreases enzyme activity by 32% Volvariella volvacea
Zn2+ 1 mM decreases enzyme activity by 67% Volvariella volvacea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min Volvariella volvacea

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ is required for catalysis. Enzyme activity increases by 54% on addition of 0.1 mM Volvariella volvacea
Mg2+ is required for catalysis. 1-10 mM slightly enhance enzyme activity by 11-12% Volvariella volvacea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28760
-
calculated from sequence Volvariella volvacea
29000
-
x * 29000, SDS-PAGE Volvariella volvacea

Organism

Organism UniProt Comment Textmining
Volvariella volvacea B8YIE0 V14
-
Volvariella volvacea V14 B8YIE0 V14
-

Purification (Commentary)

Purification (Comment) Organism
AXEII with the 6-histidine tag purified by affinity chromatography using Ni-NTA column Volvariella volvacea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium 4-day-old cultures Volvariella volvacea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min: 3448 IU/micromol with acetylated xylan as substrate, 830.9 IU/micromol with glycol chitin as substrate, 306.7 IU/micromol with chitosan as substrate, 361.1 IU/micromol with colloidal chitin as substrate Volvariella volvacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylated chitosan + H2O
-
Volvariella volvacea ? + acetate
-
?
acetylated chitosan + H2O
-
Volvariella volvacea V14 ? + acetate
-
?
acetylated colloidal chitin + H2O
-
Volvariella volvacea ? + acetate
-
?
acetylated colloidal chitin + H2O
-
Volvariella volvacea V14 ? + acetate
-
?
acetylated glycol chitin + H2O
-
Volvariella volvacea ? + acetate
-
?
acetylated glycol chitin + H2O
-
Volvariella volvacea V14 ? + acetate
-
?
acetylated xylan + H2O exhibits activity toward acetylated oat spelt xylan Volvariella volvacea xylan + acetate
-
?
acetylated xylan + H2O exhibits activity toward acetylated oat spelt xylan Volvariella volvacea V14 xylan + acetate
-
?
additional information exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate Volvariella volvacea ?
-
?
additional information exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate Volvariella volvacea V14 ?
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Volvariella volvacea

Synonyms

Synonyms Comment Organism
acetyl xylan esterase
-
Volvariella volvacea
AXEII
-
Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Volvariella volvacea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 is very stable at 60°C and, after a 6 h incubation at 50°C, over 95% of the activity remains Volvariella volvacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Volvariella volvacea

Expression

Organism Comment Expression
Volvariella volvacea axeII transcript levels are high when the fungus is grown on oat spelt xylan (highest level), cellobiose, microcrystalline cellulose, carboxymethyl-cellulose, lactose, galactose, and chitin from crab as carbon sources up