Cloned (Comment) | Organism |
---|---|
full-length cDNA or 5'-end DNA fragment subcloned into the pGEM-T vector. Full-length gene encoding the mature esterase fused with a 6-histidine tag attached at the C-terminal and ligated at the EcoR1/Xba1 sites of the Picchia pastoris expression vector pPICZaA to yield the construct pPICZaA-AXEII. Transformed and expressed in Pichia pastoris | Volvariella volvacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits at higher (10 mM) concentrations | Volvariella volvacea | |
Cu2+ | 1 mM decreases enzyme activity by 12% | Volvariella volvacea | |
EDTA | concentrations greater than 1 mM almost eliminate the activity, but simultaneous addition of Co2+ (1 mM) and Mg2+ (1 mM) restores the activity | Volvariella volvacea | |
Fe2+ | 1 mM decreases enzyme activity by 59% | Volvariella volvacea | |
Mn2+ | 1 mM decreases enzyme activity by 32% | Volvariella volvacea | |
Zn2+ | 1 mM decreases enzyme activity by 67% | Volvariella volvacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min | Volvariella volvacea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | is required for catalysis. Enzyme activity increases by 54% on addition of 0.1 mM | Volvariella volvacea | |
Mg2+ | is required for catalysis. 1-10 mM slightly enhance enzyme activity by 11-12% | Volvariella volvacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28760 | - |
calculated from sequence | Volvariella volvacea |
29000 | - |
x * 29000, SDS-PAGE | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | B8YIE0 | V14 | - |
Volvariella volvacea V14 | B8YIE0 | V14 | - |
Purification (Comment) | Organism |
---|---|
AXEII with the 6-histidine tag purified by affinity chromatography using Ni-NTA column | Volvariella volvacea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | 4-day-old cultures | Volvariella volvacea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min: 3448 IU/micromol with acetylated xylan as substrate, 830.9 IU/micromol with glycol chitin as substrate, 306.7 IU/micromol with chitosan as substrate, 361.1 IU/micromol with colloidal chitin as substrate | Volvariella volvacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated chitosan + H2O | - |
Volvariella volvacea | ? + acetate | - |
? | |
acetylated chitosan + H2O | - |
Volvariella volvacea V14 | ? + acetate | - |
? | |
acetylated colloidal chitin + H2O | - |
Volvariella volvacea | ? + acetate | - |
? | |
acetylated colloidal chitin + H2O | - |
Volvariella volvacea V14 | ? + acetate | - |
? | |
acetylated glycol chitin + H2O | - |
Volvariella volvacea | ? + acetate | - |
? | |
acetylated glycol chitin + H2O | - |
Volvariella volvacea V14 | ? + acetate | - |
? | |
acetylated xylan + H2O | exhibits activity toward acetylated oat spelt xylan | Volvariella volvacea | xylan + acetate | - |
? | |
acetylated xylan + H2O | exhibits activity toward acetylated oat spelt xylan | Volvariella volvacea V14 | xylan + acetate | - |
? | |
additional information | exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate | Volvariella volvacea | ? | - |
? | |
additional information | exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate | Volvariella volvacea V14 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Volvariella volvacea |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Volvariella volvacea |
AXEII | - |
Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Volvariella volvacea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | is very stable at 60°C and, after a 6 h incubation at 50°C, over 95% of the activity remains | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Volvariella volvacea |
Organism | Comment | Expression |
---|---|---|
Volvariella volvacea | axeII transcript levels are high when the fungus is grown on oat spelt xylan (highest level), cellobiose, microcrystalline cellulose, carboxymethyl-cellulose, lactose, galactose, and chitin from crab as carbon sources | up |