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Literature summary for 3.1.1.72 extracted from
- Expression and characterization of a thermostable acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis involved in the degradation of insoluble cellulose acetate (2013), Biosci. Biotechnol. Biochem., 77, 2495-2498.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TTE0866, DNA and amino acid seuence determination and analysis, recombinant expression of His-tagged enzyme | Caldanaerobacter subterraneus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PMSF | complete inhibition | Caldanaerobacter subterraneus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity is not affected by presence of Mg2+, Ca2+, Mn2+, Fe2+, Fe3+, Co2+, Cu2+, and Zn2+ | Caldanaerobacter subterraneus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Caldanaerobacter subterraneus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldanaerobacter subterraneus | Q8RBF4 | subsp. tengcongensis, gene TTE0866 | - |
| Caldanaerobacter subterraneus DSM 15242 | Q8RBF4 | subsp. tengcongensis, gene TTE0866 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme by nickel affinity chromatography | Caldanaerobacter subterraneus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 104 | - |
pH 8.0, 60°C | Caldanaerobacter subterraneus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Caldanaerobacter subterraneus | 1-naphthol + acetate | - |
? | |
| 1-naphthyl acetate + H2O | - |
Caldanaerobacter subterraneus DSM 15242 | 1-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Caldanaerobacter subterraneus | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Caldanaerobacter subterraneus DSM 15242 | 2-naphthol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Caldanaerobacter subterraneus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Caldanaerobacter subterraneus DSM 15242 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Caldanaerobacter subterraneus | 4-nitrophenol + propionate | - |
? | |
| cellobiose octaacetate + H2O | - |
Caldanaerobacter subterraneus | ? | - |
? | |
| cellulose acetate + H2O | different samples, CA0.88 is the best substrate, efficient complete degradation when the enzyme is mixed with a cellulolytic endo-1,4-beta-glucanase, EC 3.2.1.4 | Caldanaerobacter subterraneus | ? | - |
? | |
| glucose pentaacetate + H2O | - |
Caldanaerobacter subterraneus | ? | - |
? | |
| additional information | the enzyme shows regioselective specific activity with acetylated saccharides | Caldanaerobacter subterraneus | ? | - |
? | |
| additional information | the enzyme shows regioselective specific activity with acetylated saccharides | Caldanaerobacter subterraneus DSM 15242 | ? | - |
? | |
| sucrose octaacetate + H2O | - |
Caldanaerobacter subterraneus | ? | - |
? | |
| xylose tetraacetate + H2O | - |
Caldanaerobacter subterraneus | ? | - |
? | |
| xylose tetraacetate + H2O | - |
Caldanaerobacter subterraneus DSM 15242 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Caldanaerobacter subterraneus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CA esterase | - |
Caldanaerobacter subterraneus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Caldanaerobacter subterraneus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme, stable up to | Caldanaerobacter subterraneus |
| 70 | - |
purified recombinant enzyme, half-life is 40 min, unstable above | Caldanaerobacter subterraneus |
| 75 | - |
purified recombinant enzyme, half-life is 30 min | Caldanaerobacter subterraneus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Caldanaerobacter subterraneus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | purified recombinant enzyme, 4°C, 24 h, 80% of maximal activity within this range | Caldanaerobacter subterraneus |
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