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Literature summary for 3.1.1.72 extracted from
- Purification and characterization of an acetyl xylan esterase from Bacillus pumilus (1998), Appl. Environ. Microbiol., 64, 789-792.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bacillus pumilus |  |
| Ca2+ | - |
Bacillus pumilus | |
| Co2+ | - |
Bacillus pumilus | |
| Cu2+ | - |
Bacillus pumilus | |
| Fe2+ | - |
Bacillus pumilus | |
| Mn2+ | - |
Bacillus pumilus | |
| Zn2+ | - |
Bacillus pumilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.54 | - |
1-naphthyl acetate | - |
Bacillus pumilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus pumilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * or 5 * 40000, SDS-PAGE | Bacillus pumilus |
| 40000 | - |
5 * or 4 * 40000, SDS-PAGE | Bacillus pumilus |
| 190000 | - |
gel filtration | Bacillus pumilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus pumilus | induced by corncob powder and xylan | ? | - |
? | |
| additional information | Bacillus pumilus PS214 | induced by corncob powder and xylan | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
- |
- |
| Bacillus pumilus PS214 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus pumilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 118.8 | - |
- |
Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Bacillus pumilus | naphthol + acetate | - |
? | |
| 1-naphthyl acetate + H2O | - |
Bacillus pumilus PS214 | naphthol + acetate | - |
? | |
| 4-methylumbelliferyl acetate | - |
Bacillus pumilus | acetate + 4-methylumbelliferol | - |
? | |
| 4-methylumbelliferyl acetate | - |
Bacillus pumilus PS214 | acetate + 4-methylumbelliferol | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Bacillus pumilus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Bacillus pumilus PS214 | 4-nitrophenol + acetate | - |
? | |
| acetylxylan | - |
Bacillus pumilus | acetate + ? | - |
? | |
| acetylxylan | - |
Bacillus pumilus PS214 | acetate + ? | - |
? | |
| additional information | induced by corncob powder and xylan | Bacillus pumilus | ? | - |
? | |
| additional information | induced by corncob powder and xylan | Bacillus pumilus PS214 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * or 4 * 40000, SDS-PAGE | Bacillus pumilus |
| tetramer | 4 * or 5 * 40000, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable | Bacillus pumilus |
| 60 | - |
half-life: 1 h | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus pumilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | stable | Bacillus pumilus |
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