Application | Comment | Organism |
---|---|---|
degradation | presence of the enzyme increases the activity of alpha-glucuronidases from families GH67 and GH115 on xylan by five and nine times, respectively | Flavobacterium johnsoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
4-methylumbelliferyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae | |
0.9 | - |
4-nitrophenyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium johnsoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Flavobacterium johnsoniae | 4-methylumbelliferol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Flavobacterium johnsoniae | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Flavobacterium johnsoniae | 4-nitrophenol + butanoate | - |
? | |
4-O-methyl-glucuronoxylan + H2O | main hemicellulose in deciduous trees, and contains beta-(1,4)-linked xylopyranosyl backbone units that can be acetylated and/or substituted by alpha-(1,2)-linked 4-O-methylglucopyranosyluronic acid | Flavobacterium johnsoniae | ? + acetate | - |
? | |
feruloylated (3-O-Ara)2-O-acetyl-Xyl in xylooligomer + H2O | - |
Flavobacterium johnsoniae | feruloylated (3-O-Ara)-Xyl in xylooligomer + acetate | - |
? | |
additional information | no activity on alkyl esters longer than butanoate | Flavobacterium johnsoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45200, calculated from sequence, x * 45000, SDS-PAGE | Flavobacterium johnsoniae |
Synonyms | Comment | Organism |
---|---|---|
DFP58_4623 | - |
Flavobacterium johnsoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 16 h, 10% residual activity | Flavobacterium johnsoniae |
50 | - |
10 min, 50% residual activity | Flavobacterium johnsoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95.1 | - |
4-methylumbelliferyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae | |
120.8 | - |
4-nitrophenyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | 16 h, more than 80% residual activity | Flavobacterium johnsoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
114.7 | - |
4-methylumbelliferyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae | |
127.9 | - |
4-nitrophenyl acetate | pH 7.0, 30°C | Flavobacterium johnsoniae |