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Literature summary for 3.1.1.72 extracted from

  • Razeq, F.; Jurak, E.; Stogios, P.; Yan, R.; Tenkanen, M.; Kabel, M.; Wang, W.; Master, E.
    A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans (2018), Biotechnol. Biofuels, 11, 74 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation presence of the enzyme increases the activity of alpha-glucuronidases from families GH67 and GH115 on xylan by five and nine times, respectively Flavobacterium johnsoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
4-methylumbelliferyl acetate pH 7.0, 30°C Flavobacterium johnsoniae
0.9
-
4-nitrophenyl acetate pH 7.0, 30°C Flavobacterium johnsoniae

Organism

Organism UniProt Comment Textmining
Flavobacterium johnsoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
Flavobacterium johnsoniae 4-methylumbelliferol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Flavobacterium johnsoniae 4-nitrophenol + acetate
-
?
4-nitrophenyl butanoate + H2O
-
Flavobacterium johnsoniae 4-nitrophenol + butanoate
-
?
4-O-methyl-glucuronoxylan + H2O main hemicellulose in deciduous trees, and contains beta-(1,4)-linked xylopyranosyl backbone units that can be acetylated and/or substituted by alpha-(1,2)-linked 4-O-methylglucopyranosyluronic acid Flavobacterium johnsoniae ? + acetate
-
?
feruloylated (3-O-Ara)2-O-acetyl-Xyl in xylooligomer + H2O
-
Flavobacterium johnsoniae feruloylated (3-O-Ara)-Xyl in xylooligomer + acetate
-
?
additional information no activity on alkyl esters longer than butanoate Flavobacterium johnsoniae ?
-
?

Subunits

Subunits Comment Organism
? x * 45200, calculated from sequence, x * 45000, SDS-PAGE Flavobacterium johnsoniae

Synonyms

Synonyms Comment Organism
DFP58_4623
-
Flavobacterium johnsoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 16 h, 10% residual activity Flavobacterium johnsoniae
50
-
10 min, 50% residual activity Flavobacterium johnsoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
95.1
-
4-methylumbelliferyl acetate pH 7.0, 30°C Flavobacterium johnsoniae
120.8
-
4-nitrophenyl acetate pH 7.0, 30°C Flavobacterium johnsoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5 16 h, more than 80% residual activity Flavobacterium johnsoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
114.7
-
4-methylumbelliferyl acetate pH 7.0, 30°C Flavobacterium johnsoniae
127.9
-
4-nitrophenyl acetate pH 7.0, 30°C Flavobacterium johnsoniae