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Literature summary for 3.1.1.72 extracted from

  • Colombres, M.; Garate, J.A.; Lagos, C.F.; Araya-Secchi, R.; Norambuena, P.; Quiroz, S.; Larrondo, L.; Perez-Acle, T.; Eyzaguirre, J.
    An eleven amino acid residue deletion expands the substrate specificity of acetyl xylan esterase II (AXE II) from Penicillium purpurogenum (2008), J. Comput. Aided Mol. Des., 22, 19-28.
    View publication on PubMed
Search for more literature for 3.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant protein is expressed in Aspergillus nidulans A722 Talaromyces purpureogenus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure is determined by X-ray crystallography at 0.9 resolution Talaromyces purpureogenus

Protein Variants

Protein Variants Comment Organism
additional information preparation of a deletion mutant where the amino acid 104-114 are eliminated. This mutant is catalytically active and can hydrolyze long chain fatty acid esters with much greater efficiency than the wild type AXE II Talaromyces purpureogenus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21900
-
 x * 21900, mutant enzyme, SDS-PAGE Talaromyces purpureogenus

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus O59893 AXE2 precursor
-

Purification (Commentary)

Purification (Comment) Organism
-
 Talaromyces purpureogenus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Talaromyces purpureogenus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Talaromyces purpureogenus 1-naphthol + acetate
-
 ?
1-naphthyl caprate + H2O
-
 Talaromyces purpureogenus 1-naphthol + caprate
-
 ?
1-naphthyl myristate + H2O
-
 Talaromyces purpureogenus 1-naphthol + myristate
-
 ?

Subunits

Subunits Comment Organism
? x * 21900, mutant enzyme, SDS-PAGE Talaromyces purpureogenus

Synonyms

Synonyms Comment Organism
acetyl xylan esterase II
-
 Talaromyces purpureogenus
AXE II
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 mutant enzyme Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 mutant enzyme is less stable than the native recombinant protein at 70°C Talaromyces purpureogenus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces purpureogenus mutant enzyme, isoelectric focusing
-
 5.9
Talaromyces purpureogenus recombinant native enzyme
-
 6.8