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2.1.2.1: glycine hydroxymethyltransferase

This is an abbreviated version!
For detailed information about glycine hydroxymethyltransferase, go to the full flat file.

Word Map on EC 2.1.2.1

Reaction

5,10-methylenetetrahydrofolate
+
glycine
 +
H2O
=
tetrahydrofolate
 +
L-serine

Synonyms

AtSHMT3, bsSHMT, bstSHMT, EC 4.1.2.6, eSHMT, GlyA, GlyA protein, glycine hydroxymethyltransferrase, hSHMT, L-serine hydroxymethyltransferase, mitochondrial serine hydroxymethyltransferase, MJ1597, PvSHMT, Rhg4, serine hydroxymethyl transferase, serine hydroxymethylase hydroxymethyltransferase, serine, serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, serine hydroxymethyltransferase 2, serine hydroxymethyltransferase 2alpha, serine transhydroxymethylase, serine:H4F hydroxymethyltransferase, serine:tetrahydrofolate hydroxymethyltransferase, SHM1, SHM2, SHMT, SHMT-1, SHMT-L, SHMT-S, SHMT08, SHMT1, SHMT2, SHMT2alpha, SHMT3, zcSHMT, zebrafish cytosolic serine hydroxymethyltransferase, zebrafish mitochondiral serine hydroxymethyltransferase, zmSHMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.1 glycine hydroxymethyltransferase

Cloned

Cloned on EC 2.1.2.1 - glycine hydroxymethyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both isoforms cloned in frame with EGFP at their C-terminus and transfected into Leishmania major
-
comparison
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli resulting in the increased accumulation of glycine and L-serine. Choline and glycine betaine levels are also significantly increased. Under high salinity, the growth rate of ApSHMT-expressing cells is faster compared to its respective control
Escherichia coli DH5alpha-PUC19 and Escherichia coli BL21 (DE3) used as host-vector system
-
Escherichia coli glyA gene
-
expressed as a His-tagged fusion protein in Escherichia coli
expressed as fusion protein with an N-terminal Strep-tag in Escherichia coli strain BL21 Gold
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 gold cells
expressed in Escherichia coli BL21(DE3) and GS245(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-TIR cells
expressed in Escherichia coli DE3 cells
expressed in Escherichia coli GS245(DE3)pLysS cells
-
expressed in Escherichia coli SG 13009 cells
expressed in Escherichia coli strain GS1993 recA-
expressed in Synechococcus elongatus strain PCC7942
-
expression in Escherichia coli
expression in Escherichia coli strain BL21 (DE3)
-
expression of a dominant negative, tetracycline-inducible SHMT1 protein, DN2-SHMT1, in HeLa and SH-SY5Y cells, expression of RFP-tagged SHMT2alpha and of YFP- or FLAG-tagged SHMT1
-
expression of wild-type and chimeric mutant in Escherichia coli strain BL21 (DE3)
expression pattern of SHMT2 in 76 breast cancer patients after modified radical mastectomy, expression analysis
-
full-length cDNA clone SST18 encoding potato SHMT inserted in antisense orientation into the pBIN19 based vector L700 and introduced into the genome of Solanum tuberosum
-
gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene glyA, DNa and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, sequence comparison, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
gene glyA, DNA and amino acid sequence determination and analysis, thermal asymmetric interlaced PCR, TAIL-PCR, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene glyA, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain HMS174 (DE3)
gene HTH1832, overexpression in Escherichia coli
-
gene SHMT2, expression analysis
genes pfshmt and PF14_ 0534, encoding isozymes PfSHMTc and PfSHMTm, respectively, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged full-length isozymes
-
heterologously overproduced in Escherichia coli without a His6-tag
His6-tagged enzyme is expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
into pET43.1a vector and transformed into Escherichia coli HMS174(DE3) cells
into pQE60 vector having C terminal His6-tag, expression in Escherichia coli M15 cells
into the pGL3 vector and transfected into MCF-7 cells
-
mutations introduced into the cDNA for human cytosolic SHMT and expressed from an Escherichia coli expression system
-
mutations introduced into the cDNA for rabbit cytosolic SHMT and expressed from an Escherichia coli expression system
phylogenetic analysis, expression in Escherichia coli strain Rosetta
-
phylogenetic analysis, expression in Escherichia coli strain Rosetta, transient expression of EGFP-tagged full-length AtSHMT3 in Arabidopsis thaliana leaf protoplasts
recombinant expression in Escherichia coli strain BL21(DE3)
-
recombinant soluble enzyme overexpression in Escherichia coli strain BL21(DE3)
using the AlkS/PalkB-expression system Escherichia coli’s serine hydroxymethyltransferase gene glyA is overexpressed in Escherichia coli. It is shown that the system is already fully turned on at inducer concentrations as low as 0.005% (v/v). The optimum induction procedure for production of serine hydroxymethyltransferase is elaborated. Volumetric and specific productivity are found to increase with specific growth rate in glucose-limited fed-batch cultures. Acetate excretion as a result of recombinant protein production can be avoided in an optimized fermentation protocol by switching earlier to a linear feed. A final cell dry weight (CDW) concentration of 52 g/l is reached producing recombinant GlyA with a maximum specific activity of 6.3 U/mg total protein
-
wild-type and mutant enzymes S52A, S52C and R262A are overexpressed in Escherichia coli
-
wild-type and mutants cloned into vector pET-29B and expressed in Escherichia coli BL21(DE3) cells, moreover expressed in Trichomonas vaginalis with a C-terminal HA tag
-