Application | Comment | Organism |
---|---|---|
medicine | SHMT is a potential target for antimalarial chemotherapy | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
His6-tagged enzyme is expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Plasmodium falciparum |
General Stability | Organism |
---|---|
the kcat value of the enzyme in phosphate buffer is about 60% of that measured in HEPES buffer | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycine | glycine inhibits His6-tagged SHMT competitively with respect to serine and non-competitively with respect to tetrahydrofolate | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
D-serine | apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C | Plasmodium falciparum | |
0.193 | - |
tetrahydrofolate | in 50 mM HEPES buffer, pH 7.45, at 25°C | Plasmodium falciparum | |
0.251 | - |
L-serine | in phosphate buffer, pH 7.45, at 25°C | Plasmodium falciparum | |
0.253 | - |
tetrahydrofolate | in phosphate buffer, at 25°C | Plasmodium falciparum | |
0.371 | - |
L-serine | in 50 mM HEPES buffer, pH 7.45, at 25°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, calculated from amino acid sequence | Plasmodium falciparum |
85000 | - |
native enzyme, SDS-PAGE | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
strainTM4 | - |
Plasmodium falciparum TM4 | - |
strainTM4 | - |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography, Sephadex G-25 gel filtration, and SP-Sepharose column chromatography | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine + tetrahydrofolate | the catalytic efficiency for D-serine is 580fold lower than that of L-serine | Plasmodium falciparum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
D-serine + tetrahydrofolate | the catalytic efficiency for D-serine is 580fold lower than that of L-serine | Plasmodium falciparum TM4 | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | only (6S)-tetrahydrofolate can serve as a substrate for His6-tagged SHMT, the presence of (6R)-tetrahydrofolate has no interference to the reaction | Plasmodium falciparum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | only (6S)-tetrahydrofolate can serve as a substrate for His6-tagged SHMT, the presence of (6R)-tetrahydrofolate has no interference to the reaction | Plasmodium falciparum TM4 | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55000, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Plasmodium falciparum |
SHMT | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.246 | - |
D-serine | apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C | Plasmodium falciparum | |
0.43 | - |
L-serine | in phosphate buffer, pH 7.45, at 25°C | Plasmodium falciparum | |
0.74 | - |
L-serine | in 50 mM HEPES buffer, pH 7.45, at 25°C | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00346 | - |
D-serine | apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C | Plasmodium falciparum | |
1.99 | - |
L-serine | in 50 mM HEPES buffer, pH 7.45, at 25°C | Plasmodium falciparum |