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Literature summary for 2.1.2.1 extracted from

  • Jiang, W.; Xia, B.; Huang, J.; Liu, Z.
    Characterization of a serine hydroxymethyltransferase for L-serine enzymatic production from Pseudomonas plecoglossicida (2013), World J. Microbiol. Biotechnol., 29, 2067-2076.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Pseudomonas plecoglossicida

Inhibitors

Inhibitors Comment Organism Structure
Co2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
CTAB
-
Pseudomonas plecoglossicida
Cu2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
Fe2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
Hg2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
Mn2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
SDS
-
Pseudomonas plecoglossicida

Metals/Ions

Metals/Ions Comment Organism Structure
additional information K+, Na+, NH4+, Mg2+, Ca2+, Zn2+ and Pb2+ show no significant effect on enzyme activity Pseudomonas plecoglossicida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44500
-
x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida
60000
-
x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Pseudomonas plecoglossicida
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Pseudomonas plecoglossicida MCCC 1K00022
-
tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine Pseudomonas plecoglossicida
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine Pseudomonas plecoglossicida MCCC 1K00022
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas plecoglossicida L7Y8B0 gene glyA
-
Pseudomonas plecoglossicida MCCC 1K00022 L7Y8B0 gene glyA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Pseudomonas plecoglossicida tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Pseudomonas plecoglossicida MCCC 1K00022 tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine
-
Pseudomonas plecoglossicida 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine
-
Pseudomonas plecoglossicida MCCC 1K00022 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Subunits

Subunits Comment Organism
? x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Pseudomonas plecoglossicida
SHMT
-
Pseudomonas plecoglossicida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas plecoglossicida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 45 60% of maximal activity at 30°C and 45°C, rapid loss of activity above Pseudomonas plecoglossicida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas plecoglossicida

pH Range

pH Minimum pH Maximum Comment Organism
5.8 9 almost inactive at pH 5.8, less than 10% of maximal activity at pH 6.0, maximal activity at pH 8.0, 80% of maximal activity at pH 7.5 and pH 8.5, inactive at pH 9.5 Pseudomonas plecoglossicida

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Pseudomonas plecoglossicida
pyridoxal 5'-phosphate dependent on Pseudomonas plecoglossicida
tetrahydrofolate
-
Pseudomonas plecoglossicida