Cloned (Comment) | Organism |
---|---|
gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) | Pseudomonas plecoglossicida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong inhibition at 1 mM | Pseudomonas plecoglossicida | |
CTAB | - |
Pseudomonas plecoglossicida | |
Cu2+ | strong inhibition at 1 mM | Pseudomonas plecoglossicida | |
Fe2+ | strong inhibition at 1 mM | Pseudomonas plecoglossicida | |
Hg2+ | strong inhibition at 1 mM | Pseudomonas plecoglossicida | |
Mn2+ | strong inhibition at 1 mM | Pseudomonas plecoglossicida | |
SDS | - |
Pseudomonas plecoglossicida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | K+, Na+, NH4+, Mg2+, Ca2+, Zn2+ and Pb2+ show no significant effect on enzyme activity | Pseudomonas plecoglossicida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44500 | - |
x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE | Pseudomonas plecoglossicida |
60000 | - |
x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE | Pseudomonas plecoglossicida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Pseudomonas plecoglossicida | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Pseudomonas plecoglossicida MCCC 1K00022 | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Pseudomonas plecoglossicida | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | Pseudomonas plecoglossicida MCCC 1K00022 | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas plecoglossicida | L7Y8B0 | gene glyA | - |
Pseudomonas plecoglossicida MCCC 1K00022 | L7Y8B0 | gene glyA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pseudomonas plecoglossicida | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pseudomonas plecoglossicida MCCC 1K00022 | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Pseudomonas plecoglossicida | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | - |
Pseudomonas plecoglossicida MCCC 1K00022 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE | Pseudomonas plecoglossicida |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Pseudomonas plecoglossicida |
SHMT | - |
Pseudomonas plecoglossicida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas plecoglossicida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 45 | 60% of maximal activity at 30°C and 45°C, rapid loss of activity above | Pseudomonas plecoglossicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas plecoglossicida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9 | almost inactive at pH 5.8, less than 10% of maximal activity at pH 6.0, maximal activity at pH 8.0, 80% of maximal activity at pH 7.5 and pH 8.5, inactive at pH 9.5 | Pseudomonas plecoglossicida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Pseudomonas plecoglossicida | |
pyridoxal 5'-phosphate | dependent on | Pseudomonas plecoglossicida | |
tetrahydrofolate | - |
Pseudomonas plecoglossicida |