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2.1.2.1: glycine hydroxymethyltransferase

This is an abbreviated version!
For detailed information about glycine hydroxymethyltransferase, go to the full flat file.

Word Map on EC 2.1.2.1

Reaction

5,10-methylenetetrahydrofolate
+
glycine
 +
H2O
=
tetrahydrofolate
 +
L-serine

Synonyms

AtSHMT3, bsSHMT, bstSHMT, EC 4.1.2.6, eSHMT, GlyA, GlyA protein, glycine hydroxymethyltransferrase, hSHMT, L-serine hydroxymethyltransferase, mitochondrial serine hydroxymethyltransferase, MJ1597, PvSHMT, Rhg4, serine hydroxymethyl transferase, serine hydroxymethylase hydroxymethyltransferase, serine, serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, serine hydroxymethyltransferase 2, serine hydroxymethyltransferase 2alpha, serine transhydroxymethylase, serine:H4F hydroxymethyltransferase, serine:tetrahydrofolate hydroxymethyltransferase, SHM1, SHM2, SHMT, SHMT-1, SHMT-L, SHMT-S, SHMT08, SHMT1, SHMT2, SHMT2alpha, SHMT3, zcSHMT, zebrafish cytosolic serine hydroxymethyltransferase, zebrafish mitochondiral serine hydroxymethyltransferase, zmSHMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.1 glycine hydroxymethyltransferase

Purification

Purification on EC 2.1.2.1 - glycine hydroxymethyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on L-adsorbent
-
affinity chromatography on L-adsorbent Sepharose
-
ammonium sulfate precipitation and DEAE-cellulose column chromatography
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography, gel filtration
by affinity chromatography
-
by ammonium sulfate precipitation and gel filtration, more than 95% pure
by Ni affinity chromatography, more than 98% pure
-
by Ni2+-NTA affinity chromatography, 95% pure
chimeric enzymes
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography
DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-25 gel filtration
-
gel filtration
-
gene cloned and expressed in Escherichia coli
-
glutathione Sepharose column chromatography
glutathione-Sepharose resin column chromatography and Superdex S200 gel filtration
-
heat-denaturation omitted
-
His-Trap column chromatography and Superdex 75 gel filtration
HisTrap resin column chromatography and Superdex S200 gel filtration
Ni ion affinity column chromatography
-
Ni-Sepharose column chromatography, Sephadex G-25 gel filtration, and SP-Sepharose column chromatography
-
Ni-TED column chromatography
Ni2+-affinity column chromatography and Superdex S200 gel filtration
-
Ni2+-chelating column chromatography, SP Sepharose column chromatography and Superdex S200 gel filtration
partial by chloroplast preparation
polyethyleneimine precipitation, Q-Sepharose column chromatography and Sephacyl S-200 gel filtration
-
polyethyleneimine precipitation, SP-Sepharose column chromatography and DEAE-Sepharose column chromatography
-
recombinant enzyme
recombinant enzyme 11.2fold from Escherichia coli strain BL21(DE3) to homogeneity by polyethyleneimine precipitation, anion exchange chromatography, and gel filtration
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain BL21(DE3) by polyethyleneimine precipitation, anion and cation exchange chromatography
-
recombinant enzymes by tandem affinity purification, immunoprecipitation, heat treatment,
-
recombinant GST-tagged enzyme from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography
recombinant N-terminally His-tagged enzyme from Escherichia coli strain HMS174 (DE3) by nickel affinity chromatography
the His-tagged enzyme is purified by His GraviTrap column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
-
the pyridoxal-5'-phosphate-binding domain of SHMT is purified from inclusion bodies by rapid mixing followed by MonoQ column chromatography
-
three-step purification, gel filtration, 14.8fold, more than 95% pure
using a 30 to 50% ammonium sulfate precipitation, gel filtration and chromatography on CM-Sephadex columns. From 2 liters of cells, approximately 43 mg of pure zmSHMT is obtained with an overall yield of 90%
using Ni-NTA chromatography