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Literature summary for 2.1.2.1 extracted from
- One-carbon metabolism in plants: characterization of a plastid serine hydroxymethyltransferase (2010), Biochem. J., 430, 97-105.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis, expression in Escherichia coli strain Rosetta | Hordeum vulgare |
| phylogenetic analysis, expression in Escherichia coli strain Rosetta, transient expression of EGFP-tagged full-length AtSHMT3 in Arabidopsis thaliana leaf protoplasts | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | model of uncompetitive substrate inhibition using tetrahydropteroylglutamates with different numbers of glutamate residues, overview | Arabidopsis thaliana | |
| tetrahydropteroylglutamate | - |
Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2177 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Hordeum vulgare | 9507 | - |
| chloroplast | - |
Pisum sativum | 9507 | - |
| chloroplast | AtSHMT3 | Arabidopsis thaliana | 9507 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O | Hordeum vulgare | - |
tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | Pisum sativum | - |
tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | Arabidopsis thaliana | - |
tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | Hordeum vulgare Bob | - |
tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | Pisum sativum Progress 9 | - |
tetrahydrofolate + L-serine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q94JQ3 | ecotype Columbia | - |
| Hordeum vulgare | - |
- |
- |
| Hordeum vulgare Bob | - |
- |
- |
| Pisum sativum | - |
- |
- |
| Pisum sativum Progress 9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial by chloroplast preparation | Hordeum vulgare |
| partial by chloroplast preparation | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Hordeum vulgare | - |
| leaf | - |
Pisum sativum | - |
| rosette leaf | - |
Arabidopsis thaliana | - |
| seed | germinated, high expression level of AtSHMT3 | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Hordeum vulgare | tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pisum sativum | tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arabidopsis thaliana | tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arabidopsis thaliana | tetrahydrofolate + L-serine | i.e. tetrahydropteroylglutamate, tetrahydropteroylglutamates with more than one glutamate residue are poor substrates and competitive inhibitors, overview | r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Hordeum vulgare Bob | tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pisum sativum Progress 9 | tetrahydrofolate + L-serine | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtSHMT3 | - |
Arabidopsis thaliana |
| serine hydroxymethyltransferase | - |
Hordeum vulgare |
| serine hydroxymethyltransferase | - |
Pisum sativum |
| serine hydroxymethyltransferase | - |
Arabidopsis thaliana |
| SHMT | - |
Hordeum vulgare |
| SHMT | - |
Pisum sativum |
| SHMT | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.8 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Hordeum vulgare |
| 8.5 | - |
assay at | Pisum sativum |
| 8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Hordeum vulgare | |
| pyridoxal 5'-phosphate | dependent on | Pisum sativum | |
| pyridoxal 5'-phosphate | dependent on | Arabidopsis thaliana | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.085 | - |
tetrahydropteroylglutamate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Hordeum vulgare |
| physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Pisum sativum |
| physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana | |
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