Cloned (Comment) | Organism |
---|---|
heterologously overproduced in Escherichia coli without a His6-tag | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | at concentrations above 0.015 mM, substrate inhibition is observed | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
(6S)-tetrahydrofolate | pH 7.2, 37°C | Methanosarcina barkeri | |
0.2 | - |
L-serine | pH 7.2, 37°C | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Methanosarcina barkeri |
45047 | - |
2 * 45047, calculated from sequence | Methanosarcina barkeri |
100000 | - |
gel filtration | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q46A52 | - |
- |
Methanosarcina barkeri DSM 804 | Q46A52 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
pH 7.2, 37°C, extract from Escherichia coli cells carrying the expression vector | Methanosarcina barkeri |
4.3 | - |
pH 7.2, 37°C, purified enzyme, recombinant | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-tetrahydrofolate + L-serine | the enzyme also catalyzes the formation of methylene-tetrahydromethanopterin from tetrahydromethanopterin and L-serine, albeit with a catalytic efficiency which is less than 1% of that with (6S)-tetrahydrofolate as substrate. The catalytic efficiency with methylene-tetrahydrosarcinapterin as substrate is even lower | Methanosarcina barkeri | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
? | |
(6S)-tetrahydrofolate + L-serine | the enzyme also catalyzes the formation of methylene-tetrahydromethanopterin from tetrahydromethanopterin and L-serine, albeit with a catalytic efficiency which is less than 1% of that with (6S)-tetrahydrofolate as substrate. The catalytic efficiency with methylene-tetrahydrosarcinapterin as substrate is even lower | Methanosarcina barkeri DSM 804 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
? | |
additional information | the enzyme also catalyzes the tetrahydrofolate-independent retroaldol cleavage of L-allo-threonine and L-threonine to glycine and acetaldehyde | Methanosarcina barkeri | ? | - |
? | |
additional information | the enzyme also catalyzes the tetrahydrofolate-independent retroaldol cleavage of L-allo-threonine and L-threonine to glycine and acetaldehyde | Methanosarcina barkeri DSM 804 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43000, SDS-PAGE | Methanosarcina barkeri |
homodimer | 2 * 45047, calculated from sequence | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
GlyA | - |
Methanosarcina barkeri |
serine hydroxymethyltransferase | - |
Methanosarcina barkeri |
serine:H4F hydroxymethyltransferase | - |
Methanosarcina barkeri |
serine:tetrahydrofolate hydroxymethyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Methanosarcina barkeri |