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Literature summary for 2.1.2.1 extracted from

  • Chiba, Y.; Terada, T.; Kameya, M.; Shimizu, K.; Arai, H.; Ishii, M.; Igarashi, Y.
    Mechanism for folate-independent aldolase reaction catalyzed by serine hydroxymethyltransferase (2012), FEBS J., 279, 504-514.
    View publication on PubMed
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene HTH1832, overexpression in Escherichia coli Hydrogenobacter thermophilus

Protein Variants

Protein Variants Comment Organism
E53Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Geobacillus stearothermophilus
E74Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Ovis aries
E75L site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Oryctolagus cuniculus
E75Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Oryctolagus cuniculus
H147N site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Ovis aries
Y61A site-directed mutagenesis, the bsSHMT mutant has lost aldolase activity to L-allo-threonine Geobacillus stearothermophilus
Y61F site-directed mutagenesis, the bsSHMT mutant has lost aldolase activity to L-allo-threonine Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 L-serine pH 7.5, 70°C Hydrogenobacter thermophilus
0.68
-
 glycine pH 7.5, 70°C Hydrogenobacter thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryctolagus cuniculus 5829
-
cytosol
-
 Ovis aries 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48700
-
 2 * 48700, SDS-PAGE Hydrogenobacter thermophilus
74000
-
 gel filtration Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Oryctolagus cuniculus
-
 tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O Geobacillus stearothermophilus
-
 tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O Ovis aries
-
 tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O Hydrogenobacter thermophilus
-
 tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O Hydrogenobacter thermophilus TK-6 / IAM 12695
-
 tetrahydrofolate + L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Hydrogenobacter thermophilus
-
 gene HTH1832
-
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
 gene HTH1832
-
Oryctolagus cuniculus
-
-
-
Ovis aries
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture optimum growth temperature of 70-75°C Hydrogenobacter thermophilus
-
liver
-
 Oryctolagus cuniculus
-
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Oryctolagus cuniculus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Geobacillus stearothermophilus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Ovis aries tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Hydrogenobacter thermophilus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Oryctolagus cuniculus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Geobacillus stearothermophilus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Ovis aries tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Hydrogenobacter thermophilus tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 tetrahydrofolate + L-serine
-
 ?
5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Hydrogenobacter thermophilus TK-6 / IAM 12695 tetrahydrofolate + L-serine
-
 ?
additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Oryctolagus cuniculus ?
-
 ?
additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Hydrogenobacter thermophilus ?
-
 ?
additional information the enzyme also exhibits THF-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Ovis aries ?
-
 ?
additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Hydrogenobacter thermophilus TK-6 / IAM 12695 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Geobacillus stearothermophilus
homodimer 2 * 48700, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
 Oryctolagus cuniculus
serine hydroxymethyltransferase
-
 Geobacillus stearothermophilus
serine hydroxymethyltransferase
-
 Ovis aries
serine hydroxymethyltransferase
-
 Hydrogenobacter thermophilus
SHMT
-
 Oryctolagus cuniculus
SHMT
-
 Geobacillus stearothermophilus
SHMT
-
 Ovis aries
SHMT
-
 Hydrogenobacter thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Hydrogenobacter thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme shows high thermostability Hydrogenobacter thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 glycine pH 7.5, 70°C Hydrogenobacter thermophilus
18.7
-
 L-serine pH 7.5, 70°C Hydrogenobacter thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Hydrogenobacter thermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Oryctolagus cuniculus
pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus
pyridoxal 5'-phosphate
-
 Ovis aries
pyridoxal 5'-phosphate dependent on Hydrogenobacter thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.4
-
 glycine pH 7.5, 70°C Hydrogenobacter thermophilus
66.8
-
 L-serine pH 7.5, 70°C Hydrogenobacter thermophilus