Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
General Stability | Organism |
---|---|
above 6 M urea the dichrpoic activity is reduced significantly with a transition midpoint at 2.56 M. This process is completely reversible | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
tetrahydromethanopterin | pH 7.4, 37°C | Methanocaldococcus jannaschii | |
0.8 | - |
L-Ser | pH 7.4, 37°C | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48200 | - |
2 * 48200, SDS-PAGE | Methanocaldococcus jannaschii |
98000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrahydromethanopterin + L-Ser | - |
Methanocaldococcus jannaschii | glycine + ? | - |
? | |
tetrahydropteroylglutamate + L-Ser | - |
Methanocaldococcus jannaschii | glycine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48200, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
retroaldol cleavage of L-allo-threonine | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.53 | - |
L-Ser | pH 7.4, 37°C | Methanocaldococcus jannaschii | |
3.53 | - |
tetrahydromethanopterin | pH 7.4, 37°C | Methanocaldococcus jannaschii |