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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
 +
L-cysteine
=
ADP
 +
phosphate
 +
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

Cloned

Cloned on EC 6.3.2.2 - glutamate-cysteine ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant plasmid, pGMF, containing a gamma-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, is constructed with a copper-resistance gene as the selection marker and introduced into Saccharomyces cerevisiae YSF-31. The glutathione content of the recombinant strain is 1.5fold (13.1 mg/g*dry cells) of that in the host strain
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both regulatory subunit GCLM and catalytic subunit GCLC
catalytic subunit DNA sequence determination and analysis, expression as His-tagged wild-type enzyme and mutant R127C in Rosetta cells, functional expression of wild-type and mutant enzyme in enzyme-deficient cells
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cDNA for heavy subunit expressed in Escherichia coli
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cloning and sequencing of the cDNA encoding the catalytic subunit
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coexpression of the catalytic and the regulatory subunit from 2 different plasmids in Escherichia coli BL21(DE3), intracellular assembly of the holoenzyme
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coexpression of the His-tagged catalytic and regulatory subunits in Spodoptera frugiperda Sf9 cells via baculovirus infection, formation of the holoenzyme in the cells
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DNA and amino acid sequence determination and analysis, single copy gene, genomic structure
DNA sequence determination and analysis
DNA sequence determination and analysis, 2 genes encode the 2 subunits, chromosomal mapping to 6p12 and 1p21, constitutive expression, expression of the heavy subunit alone or in combination with the light subunit in mammalian cells reveals that the regulatory subunit improves the activity, genetic regulation involving AP-1, overview, expression of several constructs in HepG2 cells, signaling for enzyme expression
-
DNA sequence determination and analysis, gene Gsc1, expression in and functional complementation of an enzyme-deficient mutant strain
DNA sequence determination and analysis, gene GSH1 maps to chromosome X, expression in and functional complementation of an enzyme-deficient mutant strain, the yAP-1 responsive element in the promotor of gene GSH1 is involved in transcription of the gene in response to exposure to cadmium or hydrogen peroxide
DNA sequence determination and analysis, genetic regulation involving AP-1, overview
-
DNA sequence determination and analysis, heavy and light subunits, overexpression of catalytic subunit and holoenzyme in Escherichia coli BL21(DE3)
DNA sequence determination and analysis, heterodimer of a catalytic subunit and a regulatory subunit, encoded by 2 genes: GLCLC for the catalytic subunit, and GLCLR for the regulatory subunit, GLCLC polymorphism and existence of 5 alleles as defined by the trinucleotide repeat, which exhibits a range of 4 to 10 uninterrupted repeat, genotyping for the repeat of 60 tumor cancer cell lines, overview
-
DNA sequence determination and analysis, mapping to chromosome 6p12, heavy and light subunits, overexpression in Escherichia coli, individual or coexpression of the 2 subunits in COS cells, expression patterns, expression of several deletion mutants created fom the 5'-flanking region of the gene in human hepatoblastoma HepG2 cells, overexpression in human leukemia HL-60 cells
DNA sequence determination and analysis, mapping to chromosome 6p12, heavy and light subunits, overexpression in Escherichia coli, individual or coexpression of the 2 subunits in COS cells, expression patterns, expression of several deletion mutants created fom the 5'-flanking region of the gene in humen hepatoblastoma HepG2 cells, overexpression in human leukemia HL-60 cells
DNA sequence determination and analysis, mapping to chromosome 9, band D-E, heavy and light subunits
DNA sequence determination and analysis, overexpression in Escherichia coli
enzyme overexpression in Populus tremula x Populus alba (INRA female clone 717 1-B4) leaf cytosol, analysis of net Cd2+ influx in association with H+/Ca2+, Cd tolerance, and the underlying molecular and physiological mechanisms, overview
expresion of His-tagged wild-type subunits GCLM and GCLC and expression of His-tagged mutants in Escherichia coli BL21(DE3)
-
expression as C-terminally His8-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3)
-
expression in 293 and H4IIE cell lines
expression in Agrostis palustris
-
expression in Escherichia coli
expression in Escherichia coli, 36-45% amino acid sequence identity with the enzymes from rat, human, Saccharomyces cerevisiae and Schizosaccharomyces pombe
-
expression in Escherichia coli, transformation into tobacco plants
expression in poplar
-
expression of a construct consisting of 3.8 kb of the enzyme's heavy subunit gene promotor in front of luciferase as well as a fragment of the 5'-flanking sequence, transient expression in COS-1 cells
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expression of both subunits in H4IIE cells via infection with adenovirus as a vector
-
expression of the C-terminally His8-tagged wild-type enzyme in Escherichia coli BL21(DE3), expression of the mutant C319A in Escherichia coli BO265
-
expression of the enzyme at high level in deletion mutant strains of GHS1 and GSH2, the latter encoding the glutathione synthetase, the GSH1 promotor is Met4-inducible and GSH repressible
-
expression of the His-tagged wild-type enzyme and mutants in Spodoptera frugiperda Sf9 cells via baculovirus infection
-
expression of wild-type and mutant C319A enzymes in Escherichia coli BL21(DE3) as His-tagged proteins
-
expression of wild-type and mutant enzymes in Escherichia coli JM109
-
expression of wild-type and mutants in Escherichia coli BL21(DE3) as C-terminally His-tagged proteins
-
gene gclC encoding the catalytic subunit, DNA and amino acid sequence determination and analysis, quantitative expression analysis
gene GCLC, DNA and amino acid sequence determination and analysis, genotyping
-
gene GCLC, semiquantitative expression analysis in endothelial cells
-
gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha
gene GSH1 or GCL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3)
gene GSH1, screening and DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21
gene gshA, DNA and amino acid sequence determination and analysis, promoter determination, and expression analysis by S1 mapping
gene gshA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene gshA, phylogenetic tree analysis of GCS lineage I, expression of C-terminally His-tagged wild-type and mutants in Escherichia coli strain BL21(DE3) or Origami from pTEF416, subcloning in Escherichia coli strain DH5alpha, complementation of Saccharomyces cerevisiae gsh deficient strain ABC1195
-
gene gshA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gshF, recombinant expression in Escherichia coli strain JM109 from plasmid pTrc99A
gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3) coexpressing Escherichia coli acetate kinase (gene ack)
gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3), coexpression with acetate kinase (gene ack) from Lactobacillus sanfranciscensis
gene gshF, recombinant expression in Saccharomyces cerevisiae strains BY4717 and BY-G from plasmid JMB125 leading to increased levels of glutathione
gene gshI with unusual initiation codon UUG, expression of wild-type and mutant enzymes in strain BH5262
-
generation of C57Bl/6 mice that conditionally overexpress glutamate-cysteine ligase
genes gclc and gclcm (Ace-gclc and Ace-gclm), encoding the catalytic and the regulatory subunits, the genes are located on different chromosomes, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR analysis enzyme expression analysis, recombinant expression of the two subunits in Saccharomyces cerevisiae in the yeast two-hybrid system
C1KBE8; C1KBE7
genes gclC and gclM, DNA and amino acid sequence determination and analysis
-
genes gclc and gclm, encoding the two different subunits, the different genes are located on separate chromosomes, quantitative RT-PCR enzyme expression analysis
P97494; O09172
genes GCLC and GCLM, expression analysis of the genes encoding both C and M subunits of GCL
-
genes gclC and gclM, genotyping and expression analysis
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genes gclC and gclM, genotyping in healthy individuals and chronic obstructive pulmonary disease patients
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genes gclC and gclM, genotyping in healthy individuals and in schizophrenia patients, overview
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genes gclc and gclm, genotyping, analysis of correlation between genotype and smoking effects, overview
-
genes GCLC and GCLM, quantitative RT-PCR expression analysis in biopsy samples from head and neck squamous cell carcinoma tissues and adjacent normal tissues
genes I79_010621 or Gclc and I79_022778 or Gclm, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis of recombinant and wild-type enzyme subunits, determination of isozyme Gclc-X2 lacking the first 5 amino acids compared to isozymes Gclc-X0 and Gclc-X1, recombinant overexpression of the enzyme subunits in a CHO-T cell line Epi-CHO, derived from CHO-K1 cells. Gclc-X2 but not Gclm overexpression increases Gcl activity
G3HIY9; G3IG96
genetic regulation involving AP-1, constitutive expression, overview
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Hepa-1 cells transfected with GCLC (catalytic subunit) and GCLM (modifier subunit) expression vectors
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nucleotide sequence is highly homologous to those of the enzymes of human liver, rat kidney and Saccharomyces cerevisiae
-
partial DNA and amino acid sequence determination and analysis
the 2 subunits are encoded by 2 different genes and are located on chromosomes 9D-E and 3HI-3, genetic regulation involving AP-1, constitutive expression, overview
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the GAL4-UAS binary transgenic system is used to generate flies overexpressing either the catalytic (GCLc) or modulatory (GCLm) subunit of this enzyme, in a global or neuronally targeted pattern
-
transfection of COV-434 granulosa tumour cell with vectors designed for the constitutive expression of Gcl catalytic subunit, Gcl modifier subunit, or both Gcl catalytic subunit and Gcl modifier subunit
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transfecttion of embryonic fibroblast from GCLC null mice and expression in Saccharomyces cerevisiae
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wild-type and mutant enzymes are expressed in Escherichia coli
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