Cloned (Comment) | Organism |
---|---|
transfecttion of embryonic fibroblast from GCLC null mice and expression in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H370L | clinically relevant mutation in catalytic subunit GCLC. Significantly lower levels of glutathione relative to that of the wild type. Compromised enzymatic activity can largely be rescued by the addition of GCLM | Homo sapiens |
P158L | clinically relevant mutation in catalytic subunit GCLC. Significantly lower levels of glutathione relative to that of the wild type, kinetic constants comparable to those of wild-type GCLC | Homo sapiens |
P414L | clinically relevant mutation in catalytic subunit GCLC. Significantly lower levels of glutathione relative to that of the wild type, most compromised mutant among those studied. Compromised enzymatic activity can largely be rescued by the addition of GCLM | Homo sapiens |
R127C | clinically relevant mutation in catalytic subunit GCLC. Significantly lower levels of glutathione relative to that of the wild type. Compromised enzymatic activity can largely be rescued by the addition of GCLM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
L-Cys | mutant R127C, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.07 | - |
L-Cys | wild-type, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.08 | - |
L-Cys | mutant P138L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.1 | - |
L-Cys | wild-type, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.12 | - |
L-Cys | mutant R127C, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.16 | - |
L-Cys | mutant H370L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.17 | - |
L-Cys | mutant P138L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.17 | - |
L-Cys | mutant P414L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.26 | - |
ATP | mutant H370L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.3 | - |
ATP | mutant P138L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.44 | - |
L-Glu | mutant H370L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.44 | - |
ATP | wild-type, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.46 | - |
L-Glu | wild-type, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.5 | - |
ATP | mutant R127C, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.65 | - |
L-Glu | mutant P138L, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
0.68 | - |
L-Glu | mutant P138L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.77 | - |
L-Glu | mutant P414L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
0.82 | - |
ATP | mutant P414L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
1.14 | - |
L-Glu | wild-type, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
1.38 | - |
L-Glu | mutant R127C, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
1.38 | - |
L-Glu | mutant R127C, CGL holoenzyme, pH 8.0, 37°C | Homo sapiens | |
2.68 | - |
ATP | wild-type, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
3.57 | - |
ATP | mutant P138L, catalytic subunit, pH 8.0, 37°C | Homo sapiens | |
4.47 | - |
ATP | mutant R127C, catalytic subunit, pH 8.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Homo sapiens | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCLC | GCL catalytic subunit | Homo sapiens |