Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a potential drug target | Onchocerca volvulus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, single copy gene, genomic structure | Onchocerca volvulus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystamine | irreversible | Onchocerca volvulus | |
L-buthionine-R,S-sulfoximine | i.e. BSO, specific, irreversible | Onchocerca volvulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | preliminary steady state kinetics | Onchocerca volvulus | |
1.2 | - |
L-glutamate | pH 8.0, 37°C | Onchocerca volvulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74200 | - |
x * 74200, amino acid sequence calculation | Onchocerca volvulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | Onchocerca volvulus | first and rate-limiting step in the glutathione biosynthesis, important for maintenance of the intracellular thiol redox status and in detoxification processes | ADP + phosphate + gamma-L-Glu-L-Cys | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Onchocerca volvulus | Q9NFN6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Onchocerca volvulus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
r | |
ATP + L-Glu + L-Cys | first and rate-limiting step in the glutathione biosynthesis, important for maintenance of the intracellular thiol redox status and in detoxification processes | Onchocerca volvulus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 74200, amino acid sequence calculation | Onchocerca volvulus |
Synonyms | Comment | Organism |
---|---|---|
gamma-GCS | - |
Onchocerca volvulus |
gamma-Glutamylcysteine synthetase | - |
Onchocerca volvulus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Onchocerca volvulus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Onchocerca volvulus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Onchocerca volvulus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
L-buthionine-R,S-sulfoximine | pH 8.0, 37°C | Onchocerca volvulus | |
0.0039 | - |
cystamine | pH 8.0, 37°C | Onchocerca volvulus |