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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
 +
L-cysteine
=
ADP
 +
phosphate
 +
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

Molecular Weight

Molecular Weight on EC 6.3.2.2 - glutamate-cysteine ligase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
104000
-
-
114000
120000
-
PAGE
140000
-
holoenzyme, gel filtration
191000
-
bifunctional enzyme gamma-glutamate-cysteine ligase-glutathione synthetase, gel filtration
220000
-
gel filtration
24000
-
1 * 74000 + 1 * 24000, SDS-PAGE. One enzyme species of MW 100000 Da detected by SDS-PAGE after cross-linking with dimethylsuberimidate
25000
27700
-
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT
28000
-
1 * 80000, about, catalytic subunit, + 1 * 28000, about, modifier subunit, SDS-PAGE
30500
1 * 72700, heavy catalytic subunit, + 1 * 30500, light regulatory subunit, SDS-PAGE
30548
-
x * 30548, calculation from nucleotide sequence
30600
1 * 72600, heavy catalytic subunit, + 1 * 30600, light regulatory subunit, SDS-PAGE
30700
1 * 72800, heavy catalytic subunit, + 1 * 30700, light regulatory subunit, SDS-PAGE
31000
32000
-
x * 72000 + x * 32000, denaturing SDS-PAGE
34000
35000
-
x * 35000, mutant enzyme modifier subunit, SDS-PAGE
45000
46000
1 * 46000, recombinant enzyme, SDS-PAGE
49300
x * 49300, catalytic subunit
54500
recombinant enzyme, gel filtration
55000
-
gel filtration
56000
-
gel filtration, native PAGE
57000
-
1 * 66000 + 1 * 57000, SDS-PAGE
58200
1 * 58200
59900
1 * 59900, catalytic unit
60000
60000 - 65000
recombinant full-length enzyme, gel filtration
62000 - 64000
-
gel filtration, ultracentrifugal analysis, SDS-PAGE after cross-linking with dimethylsuberimidate
66000
-
1 * 66000 + 1 * 57000, SDS-PAGE
70000
71400
x * 71400, catalytic subunit
72000
-
x * 72000 + x * 32000, denaturing SDS-PAGE
72600
1 * 72600, heavy catalytic subunit, + 1 * 30600, light regulatory subunit, SDS-PAGE
72700
1 * 72700, heavy catalytic subunit, + 1 * 30500, light regulatory subunit, SDS-PAGE
72800
1 * 72800, heavy catalytic subunit, + 1 * 30700, light regulatory subunit, SDS-PAGE
74000
-
1 * 74000 + 1 * 24000, SDS-PAGE. One enzyme species of MW 100000 Da detected by SDS-PAGE after cross-linking with dimethylsuberimidate
74200
x * 74200, amino acid sequence calculation
75000
77500
1 * 77500, catalytic unit
78000
-
gel filtration
78100
1 * 78100, catalytic unit
78300
x * 78300, catalytic subunit
80000
85000
-
2 * 85000, SDS-PAGE