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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
 +
L-cysteine
=
ADP
 +
phosphate
 +
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

Storage Stability

Storage Stability on EC 6.3.2.2 - glutamate-cysteine ligase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25% glycerol, 20 mM imidazole HCl buffer, pH 7.4, 1 mM EDTA, without freezing, stable for at least 1 year
-
-20°C, purified enzyme, 25% glycerol, indefinitely stable
-20°C, purified enzyme, 25% glycerol, stable for several months
-80°C, 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, can be stored for up to 6 months without significant loss of activity
-
-80°C, enzyme is very stable at
-80°C, gills and in the digestive gland frozen without buffer, at least 6 days, loss of activity compared to fresh tissues, the activity in digestive gland, but in gills, is stable after addition of mM phosphate buffer containing PMSF, L-serine borate, and acivicin
-80°C, purified recombinant GshF in 50 mM Tris-HCl, pH 7.4, containing about 100 mM NaCl could be stored for up to 6 months without significant loss of activity
-
4°C or -20°C, about 50% loss of activity after 7 days and total loss of activity after 1 month
-
4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol
-
4°C, 10 mM imidazole buffer, pH 8.4, 50 mM DTT, irreversible loss of activity after 4 h
-
4°C, more than 50% loss of activity after 48 h. Stable at -20°C
-
4°C, or in the frozen state, 25-30% loss of activity
-
4°C, or in the frozen state, stable for 2 weeks
-
4°C, purified holoenzyme, 10% loss of activity after 1 week
-
4°C, purified recombinant enzyme, 20 mM imidazole HCl buffer, pH 7.4, 1 mM EDTA, stable for at least 7 days
-
4°C, stable for 6-10 weeks
-
4°C, stable for up to 96 h
-
50% loss of activity after 6-7 days, only 5% of activity remains after 1 month, substrates protect, almost complete reactivation by addition of 2-5 mM
-
precipitated enzyme retains its full activity after preservation for one year at 5°C
-