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Literature summary for 6.3.2.2 extracted from

  • Janowiak, B.E.; Griffith, O.W.
    Glutathione synthesis in Streptococcus agalactiae. One protein accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities (2005), J. Biol. Chem., 280, 11829-11839.
    View publication on PubMed
Search for more literature for 6.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptococcus agalactiae

Inhibitors

Inhibitors Comment Organism Structure
L-buthionine-S-sulfoximine competitive with L-glutamate Streptococcus agalactiae
additional information no significant inhibition by cystamine, L-methionine-SR-sulfoximine and GSH Streptococcus agalactiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.156
-
 L-cysteine
-
 Streptococcus agalactiae
22
-
 L-glutamate
-
 Streptococcus agalactiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities, gel filtration Streptococcus agalactiae

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-gamma-GCS-GS, bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities Streptococcus agalactiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.3
-
-
 Streptococcus agalactiae

Storage Stability

Storage Stability Organism
4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol Streptococcus agalactiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DL-alpha-aminoadipate + L-cysteine about 10% of the activity with L-glutamate Streptococcus agalactiae ADP + phosphate + DL-aminoadipyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine
-
 Streptococcus agalactiae ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
monomer bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities Streptococcus agalactiae

Synonyms

Synonyms Comment Organism
gamma-GCS-GS bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities Streptococcus agalactiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.9
-
 L-buthionine-S-sulfoximine
-
 Streptococcus agalactiae