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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
 +
L-cysteine
=
ADP
 +
phosphate
 +
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

General Stability

General Stability on EC 6.3.2.2 - glutamate-cysteine ligase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of the holoenzyme and of the catalytic subunit is reduced by 20% and 10%, respectively, after 1 cycle of freezing and thawing
-
easy loss of activity on freezing at -20°C
-
enzyme is inactivated by freezing
freezing of the purified recombinant enzyme in solution results in irreversible inactivation
-
freezing, -20°C, and thawing results in 70-80% loss of activity
-
glycerol is required for enzyme stability during storage
glycerol stabilizes
-
inactivated by freezing
L-glutamate stabilizes the enzyme during purification
L-glutamate stabilizes the enzyme during purification,
Mn2+ destabilizes the enzyme during purification
optimal stabilization in presence of 10 mM Mg2+, 24% loss of activity after 12 h at 4°C without Mg2+. Mn2+ has no effect on stability
-
treatment with 50 mM DTT for more than 1 h at 4°C, leads to some loss of activity, which is partially reversible. Further treatment leads to irreversible loss of activity
-