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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
 +
L-cysteine
=
ADP
 +
phosphate
 +
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

Purification

Purification on EC 6.3.2.2 - glutamate-cysteine ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from erythrocyte
-
from erythrocyte, from malignant astrocytoma cell line, recombinant from Escherichia coli to homogeneity
from kidney, liver and erythrocytes, recombinant catalytic subunit and holoenzyme from Escherichia coli to homogeneity
from lens
-
from liver
from reproductive tissue
-
from strain JM109, to homogeneity
His6-gamma-GCS-GS, bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities
-
Ni2+ affinity chromatography
partially
recombinant enzyme
recombinant from Escherchia coli to homogeneity
recombinant His-tagged enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged holoenzyme and individual subunits from Sf9 insect cells
-
recombinant His-tagged wild-type and mutant C319A enzymes from Escherichia coli
-
recombinant HIs-tagged wild-type and mutant enzymes
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Sf9 insect cells
-
recombinant His-tagged wild-type and mutant R127C enzyme from Rosetta cells
-
recombinant holoenzyme from Escherichia coli, to homogeneity
-
recombinant mutant C319A and recombinant His8-tagged wild-type from Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli
-