Any feedback?
Literature summary for 6.3.2.2 extracted from
- Characterization of Trypanosoma brucei gamma-glutamylcysteine synthetase, an essential enzyme in the biosynthesis of trypanothione (diglutathionylspermidine) (1996), J. Biol. Chem., 271, 17485-17490.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, 36-45% amino acid sequence identity with the enzymes from rat, human, Saccharomyces cerevisiae and Schizosaccharomyces pombe | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystamine | - |
Trypanosoma brucei |  |
| glutathione | GSH | Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
ATP | - |
Trypanosoma brucei | |
| 0.24 | - |
L-Glu | - |
Trypanosoma brucei | |
| 0.69 | - |
L-Cys | - |
Trypanosoma brucei | |
| 10 | - |
L-2-aminobutanoate | - |
Trypanosoma brucei | |
| 10.4 | - |
L-2-aminobutanoate | - |
Trypanosoma brucei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | Trypanosoma brucei | enzyme catalyzes the first committed step in the biosynthesis of trypanothione, i.e. diglutathionylspermidine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-2-aminobutanoate | - |
Trypanosoma brucei | ADP + phosphate + gamma-L-Glu-2-aminobutanoate | - |
? | |
| ATP + L-Glu + L-Cys | - |
Trypanosoma brucei | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | enzyme catalyzes the first committed step in the biosynthesis of trypanothione, i.e. diglutathionylspermidine | Trypanosoma brucei | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Trypanosoma brucei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
