Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a target for development of potential therapeutic agents | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli JM109 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
22.6 mg/ml purified enzyme, wild-type or mutant, hanging drop vapour diffusion method, equal volume of protein and reservoir solution, reservoir solution: 3.9 M sodium formate, pH 7.4, equilibration with reservoir solution at 293K, X-ray diffraction structure determination and analysis at 2.8 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C106S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
C164S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
C205S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
C223S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
additional information | construction of a quadruple mutant of the enzyme termed gamma-GCS4CS | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | Escherichia coli | catalyzes the biosynthesis of the GSH precursor | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | Escherichia coli B / ATCC 11303 | catalyzes the biosynthesis of the GSH precursor | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | catalyzes the biosynthesis of the GSH precursor | Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | - |
Escherichia coli B / ATCC 11303 | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | catalyzes the biosynthesis of the GSH precursor | Escherichia coli B / ATCC 11303 | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-GCS | - |
Escherichia coli |
gamma-Glutamylcysteine synthetase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |