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Literature summary for 6.3.2.2 extracted from

  • Kumagai, H.; Nakayama, R.; Tochikura, T.
    gamma-Glutamylcysteine synthetase from Proteus mirabilis (1982), Agric. Biol. Chem., 46, 1301-1309.
No PubMed abstract available
Search for more literature for 6.3.2.2

General Stability

General Stability Organism
easy loss of activity on freezing at -20°C Proteus mirabilis
optimal stabilization in presence of 10 mM Mg2+, 24% loss of activity after 12 h at 4°C without Mg2+. Mn2+ has no effect on stability Proteus mirabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 L-Cys
-
 Proteus mirabilis
0.16
-
 ATP
-
 Proteus mirabilis
1
-
 L-2-aminobutanoate GTP Proteus mirabilis
1.2
-
 L-Glu L-2-aminobutanoate Proteus mirabilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+ inactive in absence of Mg2+, maximal activity at 10 mM Proteus mirabilis
Mn2+ can replace Mg2+ with 18% of the efficiency Proteus mirabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000 64000 gel filtration, ultracentrifugal analysis, SDS-PAGE after cross-linking with dimethylsuberimidate Proteus mirabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + L-Cys Proteus mirabilis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacterium cadaveris
-
-
-
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-
Escherichia coli Crooks
-
-
-
Escherichia coli FKU-1
-
-
-
Escherichia coli FKU-3
-
-
-
Escherichia coli FKU-8
-
-
-
Escherichia coli K-10
-
-
-
Escherichia coli S-96
-
-
-
Klebsiella aerogenes
-
-
-
Proteus mirabilis
-
-
-
Proteus vulgaris
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas schuylkilliensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus mirabilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
precipitated enzyme retains its full activity after preservation for one year at 5°C Proteus mirabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-Glu + L-Cys 8.5% of the activity relative to L-Glu Proteus mirabilis ADP + phosphate + D-Glu-L-Cys
-
 ?
ATP + L-Glu + Gly 14.5% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-Gly
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate 60% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli S-96 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli Crooks ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli K-10 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli FKU-8 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli FKU-3 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli FKU-1 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-Ala 10.9% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-Ala
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Pseudomonas fluorescens ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Klebsiella aerogenes ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Pseudomonas aeruginosa ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Proteus vulgaris ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Bacterium cadaveris ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Pseudomonas schuylkilliensis ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli S-96 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli Crooks ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli K-10 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli FKU-8 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli FKU-3 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli FKU-1 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Proteus mirabilis ?
-
 ?
ATP + L-Glu + L-homocysteine 13.7% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-homocysteine
-
 ?
ATP + L-Glu + L-homoserine 17% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-homoserine
-
 ?
ATP + L-Glu + L-norvaline 14% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-norvaline
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser 14.5% of the activity relative to L-Cys Proteus mirabilis ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli S-96 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli Crooks ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli K-10 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli FKU-8 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli FKU-3 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
ATP + L-Glu + L-Ser
-
 Escherichia coli FKU-1 ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
GTP + L-Glu + L-Cys 87% of the activity relative to ATP Proteus mirabilis GDP + phosphate + gamma-L-Glu-L-Cys
-
 ?
UTP + L-Glu + L-Cys 12% of the activity relative to ATP Proteus mirabilis UDP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Proteus mirabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 35 10 min, stable Proteus mirabilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Proteus mirabilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 10 min stable Proteus mirabilis