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Literature summary for 6.3.2.2 extracted from
- Reversible dissociation of gamma-glutamylcysteine synthetase into two subunits (1984), J. Biol. Chem., 259, 9345-9347.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | whole enzyme and large subunit inhibited | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27700 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
| 73000 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM imidazole buffer, pH 8.4, 50 mM DTT, irreversible loss of activity after 4 h | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
| More | the heavy subunit contains all of the structural requirements for enzymatic activity and also for feedback inhibition by glutathione | Rattus norvegicus |
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