Cloned (Comment) | Organism |
---|---|
gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha | Streptococcus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | to convert feather hydrolysates into GSH with high values, the bifunctional glutathione synthetase, that comprises the activities of EC 6.3.2.2 and EC 6.3.2.3, encoded by gcsgs from Streptococcus thermophilus is surface displayed on Saccharomyces cerevisiae strain EBY100, the potential in glutathione (GSH) production from feather hydrolysates is analyzed. The surface-displayed GCSGS can be used to convert feather hydrolysates into GSH. 10 g/l of feather are converted into 321.8 mg/l GSH by the Trichoderma atroviride F6, with feather degrading ability, and surface-displayed GCSGS. Method optimization, overview | Streptococcus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Streptococcus thermophilus | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus thermophilus | D7P1H2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | - |
Streptococcus thermophilus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional glutathione synthetase | - |
Streptococcus thermophilus |
GCSGS | - |
Streptococcus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae | Streptococcus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae | Streptococcus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus thermophilus |