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Literature summary for 6.3.2.2 extracted from

  • Qiu, Z.; Tan, H.; Zhou, S.; Cao, L.
    Surface display of a bifunctional glutathione synthetase on Saccharomyces cerevisiae for converting chicken feather hydrolysate into glutathione (2014), Mol. Biotechnol., 56, 726-730 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha Streptococcus thermophilus

Protein Variants

Protein Variants Comment Organism
additional information to convert feather hydrolysates into GSH with high values, the bifunctional glutathione synthetase, that comprises the activities of EC 6.3.2.2 and EC 6.3.2.3, encoded by gcsgs from Streptococcus thermophilus is surface displayed on Saccharomyces cerevisiae strain EBY100, the potential in glutathione (GSH) production from feather hydrolysates is analyzed. The surface-displayed GCSGS can be used to convert feather hydrolysates into GSH. 10 g/l of feather are converted into 321.8 mg/l GSH by the Trichoderma atroviride F6, with feather degrading ability, and surface-displayed GCSGS. Method optimization, overview Streptococcus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Streptococcus thermophilus
-
ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus D7P1H2
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
Streptococcus thermophilus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
bifunctional glutathione synthetase
-
Streptococcus thermophilus
GCSGS
-
Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Streptococcus thermophilus