Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Wisniewski, M.; Lapinski, M.; Zdziarska, A.; Dlugosz, E.; Baska, P.
    Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase (2014), Mol. Biochem. Parasitol., 196, 12-20 .
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for development of specific enzyme inhibitors in the treatment of ancylostomiasis Ancylostoma ceylanicum
medicine the enzyme is a target for development of a vaccination against ancylostomiasis Ancylostoma ceylanicum

Cloned(Commentary)

Cloned (Comment) Organism
genes gclc and gclcm (Ace-gclc and Ace-gclm), encoding the catalytic and the regulatory subunits, the genes are located on different chromosomes, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR analysis enzyme expression analysis, recombinant expression of the two subunits in Saccharomyces cerevisiae in the yeast two-hybrid system Ancylostoma ceylanicum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Ancylostoma ceylanicum 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ancylostoma ceylanicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Ancylostoma ceylanicum
-
ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Ancylostoma ceylanicum C1KBE8 AND C1KBE7 genes GCLC and GCLM encoding catalytic heavy chain subunit and modifier light chain subunits
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
Ancylostoma ceylanicum ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 74760, catalytic subunit, + 1 * 28510, regulatory subunit, sequence calculation Ancylostoma ceylanicum
More glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM) Ancylostoma ceylanicum

Synonyms

Synonyms Comment Organism
Ace-GCL
-
Ancylostoma ceylanicum
gamma-Glutamylcysteine synthetase
-
Ancylostoma ceylanicum
GCL
-
Ancylostoma ceylanicum
GCLC gene name, catalytic subunit Ancylostoma ceylanicum
GCLM gene name, regulatory subunit Ancylostoma ceylanicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ancylostoma ceylanicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Ancylostoma ceylanicum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Ancylostoma ceylanicum

pI Value

Organism Comment pI Value Maximum pI Value
Ancylostoma ceylanicum mature enzyme, sequence calculation
-
5.43
Ancylostoma ceylanicum catalytic subunit, sequence calculation
-
6.82

General Information

General Information Comment Organism
metabolism glutamate-cysteine ligase is one of the two enzymes involved in the synthesis of glutathione (GSH). This tripeptide is synthesized by two consecutive enzymatic reactions. Ligation of glutamate and cysteine,the rate-limiting step of GSH de novo synthesis, is catalyzed by glutamate-cysteine ligase. The following addition of glycine to the dipeptide is catalyzed by glutathione synthetase, EC 6.3.2.3 Ancylostoma ceylanicum
additional information positive interaction between two subunits of glutamate-cysteine ligase is detected using the yeast two-hybrid system. Enzyme protein structure prediction using the glutamate-cysteine ligase in complex with Mg2+ and L-glutamate, comparisons of tertiary structures, overview Ancylostoma ceylanicum
physiological function the enzyme catalyses the synthesis of gamma-glutamylcysteine, a precursor of glutathione (GSH). GSH is an important intracellular molecule that protects cells against endogenous and exogenous oxidative stress, and plays a critical role in maintaining cellular redox homeostasis Ancylostoma ceylanicum