Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for development of specific enzyme inhibitors in the treatment of ancylostomiasis | Ancylostoma ceylanicum |
medicine | the enzyme is a target for development of a vaccination against ancylostomiasis | Ancylostoma ceylanicum |
Cloned (Comment) | Organism |
---|---|
genes gclc and gclcm (Ace-gclc and Ace-gclm), encoding the catalytic and the regulatory subunits, the genes are located on different chromosomes, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR analysis enzyme expression analysis, recombinant expression of the two subunits in Saccharomyces cerevisiae in the yeast two-hybrid system | Ancylostoma ceylanicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Ancylostoma ceylanicum | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Ancylostoma ceylanicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Ancylostoma ceylanicum | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ancylostoma ceylanicum | C1KBE8 AND C1KBE7 | genes GCLC and GCLM encoding catalytic heavy chain subunit and modifier light chain subunits | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | - |
Ancylostoma ceylanicum | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 74760, catalytic subunit, + 1 * 28510, regulatory subunit, sequence calculation | Ancylostoma ceylanicum |
More | glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM) | Ancylostoma ceylanicum |
Synonyms | Comment | Organism |
---|---|---|
Ace-GCL | - |
Ancylostoma ceylanicum |
gamma-Glutamylcysteine synthetase | - |
Ancylostoma ceylanicum |
GCL | - |
Ancylostoma ceylanicum |
GCLC | gene name, catalytic subunit | Ancylostoma ceylanicum |
GCLM | gene name, regulatory subunit | Ancylostoma ceylanicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ancylostoma ceylanicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Ancylostoma ceylanicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ancylostoma ceylanicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ancylostoma ceylanicum | mature enzyme, sequence calculation | - |
5.43 |
Ancylostoma ceylanicum | catalytic subunit, sequence calculation | - |
6.82 |
General Information | Comment | Organism |
---|---|---|
metabolism | glutamate-cysteine ligase is one of the two enzymes involved in the synthesis of glutathione (GSH). This tripeptide is synthesized by two consecutive enzymatic reactions. Ligation of glutamate and cysteine,the rate-limiting step of GSH de novo synthesis, is catalyzed by glutamate-cysteine ligase. The following addition of glycine to the dipeptide is catalyzed by glutathione synthetase, EC 6.3.2.3 | Ancylostoma ceylanicum |
additional information | positive interaction between two subunits of glutamate-cysteine ligase is detected using the yeast two-hybrid system. Enzyme protein structure prediction using the glutamate-cysteine ligase in complex with Mg2+ and L-glutamate, comparisons of tertiary structures, overview | Ancylostoma ceylanicum |
physiological function | the enzyme catalyses the synthesis of gamma-glutamylcysteine, a precursor of glutathione (GSH). GSH is an important intracellular molecule that protects cells against endogenous and exogenous oxidative stress, and plays a critical role in maintaining cellular redox homeostasis | Ancylostoma ceylanicum |