Cloned (Comment) | Organism |
---|---|
gene gshI with unusual initiation codon UUG, expression of wild-type and mutant enzymes in strain BH5262 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A494G | site-specific mutagenesis, 53% increased activity compared to wild-type enzyme | Escherichia coli |
A494L | site-specific mutagenesis, 65% increased activity compared to wild-type enzyme | Escherichia coli |
A494V | site-specific mutagenesis, 66% increased activity compared to wild-type enzyme | Escherichia coli |
additional information | natural K-12 mutant B possesses a Gly at position 494 compared to Ala for the K-12 wild-type enzyme, the initiation codon exchange mutant shows increased activity | Escherichia coli |
S495T | site-specific mutagenesis, 62% increased activity compared to wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | reduced, feedback inhibition of wild-type and mutants | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | Escherichia coli | rate-limiting step in glutathione biosynthesis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12, strain W3110, gene gshI | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.031 | - |
recombinant wild-type enzyme | Escherichia coli |
0.05 | - |
recombinant mutant S495T | Escherichia coli |
0.051 | - |
recombinant mutant A494V and mutant A494L | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | rate-limiting step in glutathione biosynthesis | Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-Glutamylcysteine synthetase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |