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4.1.3.1: isocitrate lyase

This is an abbreviated version!
For detailed information about isocitrate lyase, go to the full flat file.

Word Map on EC 4.1.3.1

Reaction

isocitrate
=
succinate
 +
glyoxylate

Synonyms

AceA, acuD, citrate lyase, D-threo-isocitrate: glyoxylate lyase, EgGCE, FPICL1, ICL, ICL1, ICL2, isocitrase, isocitratase, isocitrate lyase, isocitrate lyase 1, isocitrate lyase 2, isocitric lyase, isocitritase, lyase, isocitrate, MtbIcl, PDP, petal death protein, SSO1333, threo-DS-Isocitrate glyoxylate-lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.1 isocitrate lyase

Purification

Purification on EC 4.1.3.1 - isocitrate lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose)
-
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Resource Q column chromatography
-
anion exchange chromatography (DEAE), immobilized metal ion affinity chromatography (Ni2+), gel filtration
copurified with aconitase
-
ecombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
from overproducing strain
-
glutathione agarose column chromatography
-
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography
immobilized metal ion affinity chromatography (Ni2+), tags removed
Macro-Prep High Q Support column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-Sepharose chromatography
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography, cleavage of the His-tag by thrombin, and dialysis
-
recombinant protein from cell lysate by Ni-NTA metal-affinity chromatography (elution: 400 mM imidazole) and gel filtration on Superdex 200HR 10/300 column
-
to homogeneity, by Ni2+ affinity chromatography
-
to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively
-