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Literature summary for 4.1.3.1 extracted from
- Purification and characterization of a cold-adapted isocitrate lyase and expression analysis of the cold-inducible isocitrate lyase gene from the psychrophilic bacterium Colwellia psychrerythraea (2002), Extremophiles, 6, 397-405.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.633 | - |
DL-isocitrate | 15°C, pH 6.8, Km increases with elevating temperature | Colwellia maris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can replace Mg2+ | Colwellia maris | |
| Mg2+ | 10 mM, dependent | Colwellia maris | |
| Mn2+ | can partly replace Mg2+ | Colwellia maris | |
| Ni2+ | can partly replace Mg2+ | Colwellia maris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Colwellia maris |
| 240000 | - |
gel-filtration | Colwellia maris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Colwellia maris | - |
succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia maris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Colwellia maris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.52 | - |
- |
Colwellia maris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -35°C, more than 80% of the activity remains after more than 1 year | Colwellia maris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Colwellia maris | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 64000, SDS-PAGE | Colwellia maris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Colwellia maris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
complete inactivation after 2 min | Colwellia maris |
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Release 2023.1 (January 2023)
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