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Literature summary for 4.1.3.1 extracted from
- Purification and properties of isocitrate lyase from Aspergillus nidulans, a model enzyme to study catabolite inactivation in filamentous fungi (1997), Mycol. Res., 101, 410-414.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | increase of activity | Aspergillus nidulans |  |
| dithiothreitol | optimal concentration 1 mM | Aspergillus nidulans | |
| thiol compounds | increase of activity | Aspergillus nidulans | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose-1,6-bisphosphate | - |
Aspergillus nidulans | |
| malonate | - |
Aspergillus nidulans | |
| methylenesuccinate | - |
Aspergillus nidulans | |
| oxalate | - |
Aspergillus nidulans | |
| succinate | - |
Aspergillus nidulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
threo-Ds-isocitrate | - |
Aspergillus nidulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no activity in absence of exogenous cation | Aspergillus nidulans | |
| Co2+ | Co2+ 18% as effective as Mg2+ | Aspergillus nidulans | |
| Mg2+ | required for full activity, optimal concentration 5 mM | Aspergillus nidulans | |
| Mn2+ | no activity in absence of exogenous cation | Aspergillus nidulans | |
| Mn2+ | Mn2+ 31% as effective as Mg2+ | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
4 * 59000, SDS-PAGE | Aspergillus nidulans |
| 240000 | - |
gel filtration | Aspergillus nidulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Aspergillus nidulans | glyoxylate cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Aspergillus nidulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several weeks, stable | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-isocitrate | - |
Aspergillus nidulans | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Aspergillus nidulans | succinate + glyoxylate | - |
r | |
| isocitrate | glyoxylate cycle | Aspergillus nidulans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 59000, SDS-PAGE | Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | stable for 1 h | Aspergillus nidulans |
| 50 | - |
completely inactive at temperatures above 50°C | Aspergillus nidulans |
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