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Literature summary for 4.1.3.1 extracted from

  • Polanowski, A.J.; Obendorf, R.L.
    Soybean isocitrate lyase: Purification, immunoassay and N-terminal sequence (1991), Plant Physiol. Biochem., 29, 119-129.
No PubMed abstract available
Search for more literature for 4.1.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
 DL-isocitrate
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000, calculation from amino acid sequence Glycine max
64000
-
 2 * 64000, SDS-PAGE Glycine max
130000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Glycine max glyoxylate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 soybean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.48
-
-
 Glycine max

Storage Stability

Storage Stability Organism
-12°C, 20% glycerol, 21 days, 20% loss of activity Glycine max
-12°C, 20% glycerol, 7 days, 20% loss of activity Glycine max
-12°C, without glycerol, 4 h, 100% loss of activity Glycine max
4°C, 20% glycerol, 7 days, 30% loss of activity Glycine max
4°C, without glycerol, 7 days, 50% loss of activity Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Glycine max succinate + glyoxylate
-
 ?
isocitrate glyoxylate cycle Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Glycine max
dimer 2 * 63000, calculation from amino acid sequence Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 heating to temperatures above caused loss of activity Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7 9 30% of optimal activity at pH 7 and pH 9 Glycine max