Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM 109 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
itaconic anhydride | - |
Mycobacterium tuberculosis | |
XHD-1 | extract of Illicium verum | Mycobacterium tuberculosis | |
XHD-2 | extract of Zingiber officinale | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.159 | - |
isocitrate | in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose chromatography | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
4°C, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0, 2 months, no loss of activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Mycobacterium tuberculosis | succinate + glyoxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0033 | - |
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C | Mycobacterium tuberculosis | itaconic anhydride | |
0.0182 | - |
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C | Mycobacterium tuberculosis | XHD-2 | |
0.0477 | - |
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C | Mycobacterium tuberculosis | XHD-1 |