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Literature summary for 4.1.3.1 extracted from
- Studies on isocitrate lyase isolated from Lupinus cotyledons (1979), Can. J. Biochem., 57, 1131-1137.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malonate | - |
Lupinus sp. |  |
| N-ethylmaleimide | - |
Lupinus sp. | |
| oxalate | - |
Lupinus sp. | |
| p-chloromercuribenzoate | - |
Lupinus sp. | |
| phosphoenolpyruvate | - |
Lupinus sp. | |
| succinate | - |
Lupinus sp. | |
| Tartrate | - |
Lupinus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 0.2 | isocitrate | Tris, triethanolamine or imidazole buffer | Lupinus sp. | |
| 1 | - |
isocitrate | phosphate buffer, pH 6.8 or 7.5 | Lupinus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Lupinus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 145000 | 170000 | gel filtration | Lupinus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Lupinus sp. | glyoxylate cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus sp. | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| reducing agent necessary for maintenance of enzyme activity | Lupinus sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Lupinus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.68 | - |
- |
Lupinus sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 0.3 mM dithiothreitol, 12 days, 50% loss of activity | Lupinus sp. |
| -30°C, 12 days, almost all activity lost | Lupinus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Lupinus sp. | succinate + glyoxylate | - |
r | |
| isocitrate | glyoxylate cycle | Lupinus sp. | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
triethanolamine buffer | Lupinus sp. |
| 7.5 | - |
phosphate, Tris or imidazole buffer | Lupinus sp. |
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